Kinetic evaluation and study of gold-based nanoparticles and multi-walled carbon nanotubes as an alkaline phosphatase inhibitor in serum and pure form

In this study, gold nanoparticles (AuNPs) were synthesised using green chemistry to decorate multi-walled carbon nanotubes (MWCNTs) made from walnut shells transmission electron microscopy, field-emission scanning electron microscopy (FESEM), atomic force microscopy and fourier transforms infrared s...

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Bibliographic Details
Published in:Carbon Letters 2023-06, Vol.33 (4), p.1217-1229
Main Authors: Mohammed, Samar Naser, Al-Rawi, Khalid F., Mohammed, Ahmed Mishaal
Format: Article
Language:English
Subjects:
Alkaline phosphatase
Aqueous solutions
Atomic force microscopy
Automation
Biocompatibility
Biomarkers
Carbon
Chemical synthesis
Disease
Electron microscopy
Energy dispersive X ray analysis
Enzymatic activity
Enzyme activity
Enzymes
Fourier transforms
Gold
Green chemistry
Infrared spectroscopy
Kinases
Microscopy
Multi wall carbon nanotubes
Nanoparticles
Nanotechnology
Nanotubes
Orthophosphate
Oxidation
Phosphatase
Phosphate esters
Scanning electron microscopy
Substrates
Sulfuric acid
Transmission electron microscopy
X ray analysis
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Summary:In this study, gold nanoparticles (AuNPs) were synthesised using green chemistry to decorate multi-walled carbon nanotubes (MWCNTs) made from walnut shells transmission electron microscopy, field-emission scanning electron microscopy (FESEM), atomic force microscopy and fourier transforms infrared spectroscopy were used to diagnose MWCNTs and AuNPs. MWCNT-COOAu, MWCNT-COO and MWCNT-Au were diagnosed by Raman, energy dispersive X-ray analysis and FESEM. The effect of AuNPs, MWCNT-COO, MWCNT-COOAu and MWCNT-Au on pure and serum alkaline phosphatase (ALP) enzyme activity was studied in vitro using the enzyme-substrate 4-nitrophenyl disodium orthophosphate. For pure enzymes, Vmax slightly increased as the concentration of MWCNT-Au, MWCNT-COOAu and MWCNTCOO increased, whereas the Vmax values decreased as the concentration of AuNPs increased. The inhibition type for all NPs varied. For serum ALP enzyme, the Vmax values for Au-based NPs decreased as the concentration of NPs increased. The Vmax values exceeded the standard value at the concentrations of 25, 50 and 75 ppm for MWCNT-Au and MWCNT-COOAu, whereas the Vmax values increased over the standard value for all concentrations of AuNPs.
ISSN:1976-4251
2233-4998
DOI:10.1007/s42823-023-00509-z