Formation of zinc oxide composites of doxycycline with high antibacterial activity based on DC-magnetron deposition of ZnO nanoscale particles on the drug surface

• Published in: Applied physics. A, Materials science & processing Materials science & processing, 2024-03, Vol.130 (3), Article 152
• Main Authors: Arakelova, Emma R., Khachatryan, Ashot M., Mirzoian, Anait A., Grigoryan, Susanna L., Muradyan, Rafayel E., Stepanyan, Hrachik R., Grigoryan, Stepan G., Yeranosyan, Mcrtich A., Martiryan, Armen I., Zatikyan, Ashkhen L.
• Format: Article
• Language: English
• Subjects: Characterization and Evaluation of Materials; Complex formation; Composite materials; Condensed Matter Physics; Deposition; Fourier transforms; Hydrogen bonds; In vivo methods and tests; Infrared spectroscopy; Machines; Magnetron sputtering; Manufacturing; Nanotechnology; Optical and Electronic Materials; Physics; Physics and Astronomy; Polyvinyl alcohol; Processes; Room temperature; Spectrum analysis; Substrates; Surfaces and Interfaces; Thin Films; Zinc oxide; Zinc oxides
• ISSN: 0947-8396; 1432-0630
• DOI: 10.1007/s00339-024-07296-y

Using DC-magnetron sputtering of a Zn target in an Ar: O 2 medium at 0.133 Pa pressure, with a room temperature substrate by deposition of ZnO nanoscale particles (ZnO NPs) on the surfaces of the antibiotic drug doxycycline (DOXY) in the form of a coating, DOXY, DOXY with polyvinyl alcohol (PVA) composite film (DOXY + PVA) and their zinc oxide composites (DOXY + ZnO, DOXY + PVA + ZnO) were prepared. The purpose of obtaining ZnO composites of the antibacterial drug DOXY is to enhance the antibacterial activity of the preparation. DOXY, DOXY + PVA, DOXY + ZnO and DOXY + PVA + ZnO was characterized by XRD, FTIR spectroscopy, AFM and in vitro and in vivo antibacterial studies. FTIR spectra of DOXY and its ZnO composites show the new vibration bands of OH and NH groups. The hydrogen bond between DOXY and ZnO promotes complex formation. Through AFM, FTIR and XRD studies DOXY + ZnO and DOXY + PVA + ZnO with optimal of ZnO NPs sizes were shown to have higher antibacterial activity than DOXY. In the particle size ranges of 73–112 nm on DOXY and 60–101 nm on DOXY + PVA surfaces, synergetic hydrogen bonds between ZnO NPs and DOXY contribute to the higher antibacterial activity of ZnO composites of DOXY compared with the initial DOXY. In vitro and in vivo mice studies of the DOXY, DOXY + PVA, DOXY + ZnO, and DOXY + PVA + ZnO on Staphylococcus aureus and Shigella flexneri strains  shown higher antibacterial activity of ZnO composites in the form of coating and PVA composite films lower doses of DOXY in the composition in compared with the initial drug.