Comparison of genetic diversity and phylogenetic structure of BRCA1 gene of some domestic and wild sheep breeds in different countries

BRCA1 gene plays an important role in DNA damage repair, cell cycle, and transcription process regulation; hence it's called gate keeper. The current research aims to perform bioinformatics analyzes of the BRCA1 gene of different breeds of domestic and wild sheep from 49 breeds in 14 countries...

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Bibliographic Details
Published in:Animal biotechnology 2023-12, Vol.34 (9), p.4746-4759
Main Authors: Nikmanesh, Alireza, Esmailizadeh, Ali, Asadollahpour Nanaei, Hojjat, Ezedinloo, Leila, Asadi Fozi, Masood
Format: Article
Language:English
Subjects:
Animal populations
Bioinformatics
BRCA1 gene
BRCA1 protein
Breast cancer
Cell cycle
Conserved sequence
DNA damage
DNA repair
Gene polymorphism
Gene regulation
Genetic distance
Genetic diversity
Genetic drift
Genomes
Haplotypes
Inbreeding
Nucleotides
phylogenetic structure
Phylogenetics
Phylogeny
Polymorphism
Population genetics
Sheep
Software
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Summary:BRCA1 gene plays an important role in DNA damage repair, cell cycle, and transcription process regulation; hence it's called gate keeper. The current research aims to perform bioinformatics analyzes of the BRCA1 gene of different breeds of domestic and wild sheep from 49 breeds in 14 countries using the NCBI genome database. The desired sequences were aligned using MEGA11 software and a phylogenetic tree was drawn by Neighbor-Joining method. The number of mutations, nucleotide diversity, and haploid diversity were also analyzed using Dnaspv5 software. The analyses showed 296 polymorphisms, which led to the creation of 45 different haplotypes with a haplotype diversity of 0.035. Nucleotide diversity and average nucleotide differences among breeds were estimated as 0.259 and 0.052, respectively. The average genetic distance within the population of countries was calculated as 0.052. The amount of sequence conservation in this research was 0.313 on average, which indicates the high polymorphism of this gene and the emergence of new proteins. Tajima's D value in Tajima's neutrality test was −2.421, which was significant (p 
ISSN:1049-5398
1532-2378
DOI:10.1080/10495398.2023.2187410