Terahertz Time-Domain Spectroscopic (THz-TDS) Insights into Protein Deformation

In this study, we utilized terahertz time-domain spectroscopy (THz-TDS) to study the radiation-induced protein deformation. The absorption coefficient spectra obtained from THz-TDS measurements in the frequency range (0.06–2 THz) was fitted using the Lorentzian model. The absorption coefficient fitt...

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Bibliographic Details
Published in:Brazilian journal of physics 2024-04, Vol.54 (2), Article 54
Main Authors: Abouelsayed, A., El-Bahy, Gamal S., Abdelrazzak, Abdelrazek B.
Format: Article
Language:English
Subjects:
Absorptivity
Bonding strength
Frequency ranges
General and Applied Physics
Hydrogen bonds
Permittivity
Physics
Physics and Astronomy
Polypeptides
Proteins
Radiation effects
Refractivity
Spectra
Spectrum analysis
Terahertz frequencies
Time domain analysis
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Summary:In this study, we utilized terahertz time-domain spectroscopy (THz-TDS) to study the radiation-induced protein deformation. The absorption coefficient spectra obtained from THz-TDS measurements in the frequency range (0.06–2 THz) was fitted using the Lorentzian model. The absorption coefficient fitting data was used to identify the α-helix and β-structure relative contributions in the protein secondary structure of the kidney tissue of rats irradiated with 10-cGy and 2-Gy X-ray separately or in combination. Our data show that 2-Gy X-irradiation leads to an increase in the β-structure contribution associated with a decrease in the α-helix contribution as indicated by the fitting parameters extracted from fitting the absorption coefficient α(ω) spectra with the Lorentzian function. The results point out that there is a strong correlation between the strength of the hydrogen bonds located between or inside the polypeptide chains of the extended β-sheet and α-helix, respectively, and the absolute value of the absorption coefficient α(ω), the refractive index, and the dielectric constant. The lowest refractive index and dielectric constant are recorded in the 2-Gy-irradiated group followed by the 10-cGy–2 Gy-irradiated group while the least effect was recorded in the 10-cGy-irradiated group. These data provide evidence of the adaptive effect of the 10-cGy X-irradiation delivered 24 h prior to the 2-Gy x-irradiation.
ISSN:0103-9733
1678-4448
DOI:10.1007/s13538-024-01434-w