Determination of pore edge tension from the kinetics of rupture of giant unilamellar vesicles using the Arrhenius equation: effects of sugar concentration, surface charge and cholesterol

• Published in: Physical chemistry chemical physics : PCCP 2024-02, Vol.26 (7), p.617-6117
• Main Authors: Karal, Mohammad Abu Sayem, Billah, Md. Masum, Ahamed, Md. Kabir
• Format: Article
• Language: English
• Subjects: Buffers; Charge density; Cholesterol; Kinetics; Lipid Bilayers; Lipids; Membranes; Phosphatidylcholines; Physiological effects; Physiology; Pore formation; Stability; Sugar; Sugars; Surface charge; Unilamellar Liposomes; Vesicles
• ISSN: 1463-9076; 1463-9084
• DOI: 10.1039/d3cp04451c

The pore edge tension ( Γ ) of a membrane closely intertwines with membrane stability and plays a vital role in the mechanisms that facilitate membrane resealing following pore formation caused by electrical and mechanical tensions. We have explored a straightforward procedure to determine Γ by fitting the inverse of the tension-dependent logarithm of the rate constant of rupture of giant unilamellar vesicles (GUVs) using the Arrhenius equation. The GUVs were prepared using a combination of 1,2-dioleoyl- sn-glycero -3-phospho-(1′- rac -glycerol) (DOPG) and 1, 2-dioleoyl- sn-glycero -3-phosphocholine (DOPC) in a physiological environment. The effects of sugar concentration, membrane surface charge density, and membrane cholesterol concentration on Γ have been investigated. The values of Γ increase with sugar concentration in the physiological buffer, measuring 9.6 ± 0.3, 10.4 ± 0.1, and 16.2 ± 0.1 pN for 40, 100, and 300 mM, respectively. A higher concentration of anionic lipids (70 mol% of DOPG) significantly reduces Γ . An increasing trend of Γ with cholesterol content was observed; specifically, the values of Γ were 11.9 ± 0.9, 13.9 ± 0.7, and 16.2 ± 0.4 pN for 15, 29, and 40 mol% cholesterol, respectively. Thus, the presence of higher anionic lipids in the bilayer led to a decrease in membrane stability. In contrast, the presence of higher sugar concentrations in the buffer and increased cholesterol concentration in the membranes enhanced membrane stability. Determination of membrane pore edge tension using the Arrhenius equation by varying the sugar concentration, surface charge density, and cholesterol.