Bioelectricity production of microbial fuel cells (MFCs) and the simultaneous monitoring using developed multi-channels Arduino UNO-based data logging system

• Published in: Journal of applied electrochemistry 2024-03, Vol.54 (3), p.503-518
• Main Authors: Indriyani, Yohanna Anisa, Rustami, Erus, Rusmana, Iman, Anwar, Syaiful, Djajakirana, Gunawan, Santosa, Dwi Andreas
• Format: Article
• Language: English
• Subjects: Analog to digital converters; Aquaculture; Biocatalysts; Biochemical fuel cells; Bioelectricity; Bioreactors; Channels; Chemistry; Chemistry and Materials Science; Data logging; Developing countries; Electricity generation; Electrochemical analysis; Electrochemistry; Electrode polarization; Industrial Chemistry/Chemical Engineering; LDCs; Low cost; Microorganisms; Monitoring; Multimeters; Open circuit voltage; Performance evaluation; Physical Chemistry; Research Article; Statistical analysis
• ISSN: 0021-891X; 1572-8838
• DOI: 10.1007/s10800-023-01989-7

Microbial fuel cell (MFC), a bio-electrochemical device that exploits electroactive microbes, has gained more attention in developing countries, such as Indonesia. Unfortunately, studies related to bio-electrochemistry are often constrained due to the need for precise and high-cost instrumentation, such as data logger/acquisition or data logging-multimeter for continuously monitoring electricity generation of MFCs in the rigid time interval. This present work aimed at two issues: (1) to evaluate the use of a low-cost microcontroller-based data logger, the developed multi-channels Arduino UNO-based data logging system, for monitoring the electricity generation of ten MFC bioreactors simultaneously, and (2) to evaluate the electrochemical performance of MFCs biocatalysts by ten electroactive microbes isolated from aquaculture pond sediment in Indonesia. The monitoring system worked with a multi-channels Arduino UNO, a multiplexer, an external 16-bit ADC (analogue to digital converter) ADS1115, and a RTC (real time clock) module. The MFC performance was evaluated in the terms of open circuit voltage and close circuit voltage (polarization curve, power density, and losses). Statistical analysis confirmed the high accuracy of the developed system with the average of absolute and relative error values of 1.21 mV and 1.26%, respectively, comparable to traditional multimeter utilized for MFC electricity measurement. These results suggested that the developed data logging system could be a considerable option as a low-cost monitoring device for electrochemical studies of MFCs. Electrochemical performances of ten anodic biocatalysts were also evaluated, suggesting that there were three effective bacteria (isolate KCf2, KCf4, and KCf10) for producing relatively stable bioelectricity in the reactor of MFCs. These three electroactive microbes can produce power density of 0.069 W m −2 , 0.021 W m −2 , and 0.010 W m −2 , respectively. Graphical Abstract