Expansion supercritical fluid into an aqueous solution (ESSAS), a new technique for creating nano-size cyanocobalamin-loaded liposomes, and optimization of involved parameters

Liposomes are one of the carriers used as drug delivery systems and are formed by phospholipids. They can protect the activity of the drug, increase its bioavailability and safety, and maintain the release of therapeutic molecules in the body. Cyanocobalamin is a synthetic form of vitamin B12 that r...

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Bibliographic Details
Published in:Journal of the Iranian Chemical Society 2024-02, Vol.21 (2), p.373-385
Main Authors: Mohammadi, Misagh, Karimi, Mehrnaz, Raofie, Farhad
Format: Article
Language:English
Subjects:
Analytical Chemistry
Aqueous solutions
Bioavailability
Biochemistry
Chemistry
Chemistry and Materials Science
Critical pressure
Cyanocobalamin
Drug delivery systems
Factor analysis
Inorganic Chemistry
Liposomes
Lymphocytes
Optimization
Organic Chemistry
Parameters
Phospholipids
Physical Chemistry
Polydispersity
Pressure drop
Size distribution
Storage stability
Supercritical fluids
Zeta potential
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Summary:Liposomes are one of the carriers used as drug delivery systems and are formed by phospholipids. They can protect the activity of the drug, increase its bioavailability and safety, and maintain the release of therapeutic molecules in the body. Cyanocobalamin is a synthetic form of vitamin B12 that regulates glucose metabolism, plays a role in DNA synthesis, and facilitates the production of T lymphocytes. In this study, a brand-new continuous supercritical fluid method called Expansion Supercritical Fluid into an aqueous solution suggested for creating nanometric-sized cyanocobalamin liposomes. This is a modified Expansion Supercritical Fluid method in which the pressure decreases but remains higher than the critical pressure, producing homogeneous liposomes with narrow size distribution. The single-factor analysis was used to optimize the operation parameters like equilibrium pressure, temperature, and pressure drop. Then, the storage stability and drug release of liposomes were investigated. The results demonstrated that produced liposomes at the optimal conditions showed the encapsulation efficiency, mean liposome size, zeta-potential, and polydispersity index (PDI) of the prepared liposomes were 73.6%, 96 nm, 0.264, and 35 mV, respectively. Additionally, compared to free drugs, the created liposomes were stable and could release cyanocobalamin more consistently and for more extended time.
ISSN:1735-207X
1735-2428
DOI:10.1007/s13738-023-02930-7