Polydopamine-coated photoautotrophic bacteria for improving extracellular electron transfer in living photovoltaics

• Published in: Nano research 2024-02, Vol.17 (2), p.866-874
• Main Authors: Reggente, Melania, Roullier, Charlotte, Mouhib, Mohammed, Brandl, Patricia, Wang, Hanxuan, Tacconi, Stefano, Mura, Francesco, Dini, Luciana, Labarile, Rossella, Trotta, Massimo, Fischer, Fabian, Boghossian, Ardemis A.
• Format: Article
• Language: English
• Subjects: Atomic/Molecular Structure and Spectra; Biomedicine; Biotechnology; Cell viability; Chemistry and Materials Science; Coatings; Condensed Matter Physics; Dopamine; Electrochemistry; Electrodes; Electron microscopes; Electron microscopy; Electron transfer; Fluorescence microscopy; Materials Science; Microorganisms; Microscopy; Nanoparticles; Nanotechnology; Photoelectric effect; Photovoltaic cells; Photovoltaics; Potassium chloride; Raman spectroscopy; Research Article; Scanning electron microscopy; Silver chloride; Solar energy; Solar energy conversion; Transmission electron microscopy
• ISSN: 1998-0124; 1998-0000
• DOI: 10.1007/s12274-023-6396-1

Living photovoltaics are microbial electrochemical devices that use whole cell–electrode interactions to convert solar energy to electricity. The bottleneck in these technologies is the limited electron transfer between the microbe and the electrode surface. This study focuses on enhancing this transfer by engineering a polydopamine (PDA) coating on the outer membrane of the photosynthetic microbe Synechocystis sp. PCC6803. This coating provides a conductive nanoparticle shell to increase electrode adhesion and improve microbial charge extraction. A combination of scanning electron microscopy (SEM), transmission electron microscopy (TEM), UV–Vis absorption, and Raman spectroscopy measurements were used to characterize the nanoparticle shell under various synthesis conditions. The cell viability and activity were further assessed through oxygen evolution, growth curve, and confocal fluorescence microscopy measurements. The results show sustained cell growth and detectable PDA surface coverage under slightly alkaline conditions (pH 7.5) and at low initial dopamine (DA) concentrations (1 mM). The exoelectrogenicity of the cells prepared under these conditions was also characterized through cyclic voltammetry (CV) and chronoamperometry (CA). The measurements show a three-fold enhancement in the photocurrent at an applied bias of 0.3 V (vs. Ag/AgCl [3 M KCl]) compared to non-coated cells. This study thus lays the framework for engineering the next generation of living photovoltaics with improved performances using biosynthetic electrodes.