Comparative effect of anthocyanin on proliferation and migration of human gingival fibroblasts in the absence or presence of nicotine

[...]a concentration of 200 pM significantly reduced cell viability by about 20% at all the three-time intervals (P

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Published in:Journal of periodontology and implant dentistry 2023-07, Vol.15 (2), p.100-107
Main Authors: Azimian, Sarina, Torshabi, Maryam, Esfahrood, Zeinab Rezaei
Format: Article
Language:English
Subjects:
Anthocyanins
Antibiotics
Antioxidants
Cell adhesion & migration
Cell culture
Cell growth
Cell migration
Cell viability
Chronic illnesses
Collagen
Cytotoxicity
Fibroblasts
Free radicals
Humidity
Nicotine
Oxidative stress
Signal transduction
Smoking
Toxicity
Wound healing
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creator Azimian, Sarina
Torshabi, Maryam
Esfahrood, Zeinab Rezaei
description [...]a concentration of 200 pM significantly reduced cell viability by about 20% at all the three-time intervals (P
doi_str_mv 10.34172/japicl.2023.018
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[...]the understudied CC concentrations decreased nicotine's adverse effects on cell migration to some extent. Nicotine stimulates the production of free radicals, which is associated with oxidative stress and a deficient antioxidant defense mechanism. [...]anthocyanins may protect gingival tissues against nicotine toxicity by reducing free radicals.17 Cyanidin chloride (CyCl or CC) suppresses the RANKL-stimulated nuclear factor-kappa В (NF-кВ) signaling pathway and stimulates the nuclear factor erythroid 2-related factor 2 (Nrf2) signaling pathway. According to the ISO-10-993-5 standard (2009), a group is considered cytotoxic if it reduces viability by more than 30% compared to the control group (viability rate falls below 70%).23 After determining the selected nicotine concentrations (concentrations of 1, 2, 3, 4, and 5 mM) obtained from previous studies,21122 and also selecting the appropriate concentrations of anthocyanin CC obtained from the MTT test (concentrations of 0,5,10,25 and 50 pM), in the second phase of the study, the possible antagonistic effects of the studied antioxidant were investigated. [...]HGFs (3500/well) were cultured in each well of 96-well cell culture plates on the first day at a logarithmic growth phase. Cell migration assay An in vitro scratch assay was performed to evaluate the HGF cell migration rate (which indicates the cell's ability to repair) after stimulation with a selective concentration of nicotine (2.5 mM) and in the presence and absence of three selected concentrations (10, 25, and 50 mM) of the antioxidant anthocyanin CC.22 On the first day of the study, 100000 HGFs in the logarithmic growth phase were cultured in each well of a 24-well plate.</description><identifier>ISSN: 2008-7748</identifier><identifier>EISSN: 2008-7756</identifier><identifier>DOI: 10.34172/japicl.2023.018</identifier><language>eng</language><publisher>Tabriz: Tabriz University of Medical Sciences</publisher><subject>Anthocyanins ; Antibiotics ; Antioxidants ; Cell adhesion &amp; migration ; Cell culture ; Cell growth ; Cell migration ; Cell viability ; Chronic illnesses ; Collagen ; Cytotoxicity ; Fibroblasts ; Free radicals ; Humidity ; Nicotine ; Oxidative stress ; Signal transduction ; Smoking ; Toxicity ; Wound healing</subject><ispartof>Journal of periodontology and implant dentistry, 2023-07, Vol.15 (2), p.100-107</ispartof><rights>2023. 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[...]the understudied CC concentrations decreased nicotine's adverse effects on cell migration to some extent. Nicotine stimulates the production of free radicals, which is associated with oxidative stress and a deficient antioxidant defense mechanism. [...]anthocyanins may protect gingival tissues against nicotine toxicity by reducing free radicals.17 Cyanidin chloride (CyCl or CC) suppresses the RANKL-stimulated nuclear factor-kappa В (NF-кВ) signaling pathway and stimulates the nuclear factor erythroid 2-related factor 2 (Nrf2) signaling pathway. According to the ISO-10-993-5 standard (2009), a group is considered cytotoxic if it reduces viability by more than 30% compared to the control group (viability rate falls below 70%).23 After determining the selected nicotine concentrations (concentrations of 1, 2, 3, 4, and 5 mM) obtained from previous studies,21122 and also selecting the appropriate concentrations of anthocyanin CC obtained from the MTT test (concentrations of 0,5,10,25 and 50 pM), in the second phase of the study, the possible antagonistic effects of the studied antioxidant were investigated. [...]HGFs (3500/well) were cultured in each well of 96-well cell culture plates on the first day at a logarithmic growth phase. Cell migration assay An in vitro scratch assay was performed to evaluate the HGF cell migration rate (which indicates the cell's ability to repair) after stimulation with a selective concentration of nicotine (2.5 mM) and in the presence and absence of three selected concentrations (10, 25, and 50 mM) of the antioxidant anthocyanin CC.22 On the first day of the study, 100000 HGFs in the logarithmic growth phase were cultured in each well of a 24-well plate.</description><subject>Anthocyanins</subject><subject>Antibiotics</subject><subject>Antioxidants</subject><subject>Cell adhesion &amp; migration</subject><subject>Cell culture</subject><subject>Cell growth</subject><subject>Cell migration</subject><subject>Cell viability</subject><subject>Chronic illnesses</subject><subject>Collagen</subject><subject>Cytotoxicity</subject><subject>Fibroblasts</subject><subject>Free radicals</subject><subject>Humidity</subject><subject>Nicotine</subject><subject>Oxidative stress</subject><subject>Signal transduction</subject><subject>Smoking</subject><subject>Toxicity</subject><subject>Wound healing</subject><issn>2008-7748</issn><issn>2008-7756</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><recordid>eNqNjsFOwzAMhiME0ibYnaMlzitp2q3teQLxANwnNzhtqtTpmnQSD8B7k0kVZ3zx_8uff1uI51xmRZlX6nXAyWqXKamKTOb1ndgqKet9VR2O93-6rDdiF8IgUxWNanK1FT8nP044Y7RXAjKGdARvADn2Xn8jWwbPMM3eWUM3LDnkLxhtt7pE98uIDJ3lzl7RgbHt7FuHIQZI-7EnwDYQawI_pyxatQG22kfL9CQeDLpAu7U_ipf3t8_Txz4dviwU4nnwy8xpdFa3x8vDsVHF_6hfA3lbbg</recordid><startdate>20230701</startdate><enddate>20230701</enddate><creator>Azimian, Sarina</creator><creator>Torshabi, Maryam</creator><creator>Esfahrood, Zeinab Rezaei</creator><general>Tabriz University of Medical Sciences</general><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>CWDGH</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>20230701</creationdate><title>Comparative effect of anthocyanin on proliferation and migration of human gingival fibroblasts in the absence or presence of nicotine</title><author>Azimian, Sarina ; 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subjects Anthocyanins
Antibiotics
Antioxidants
Cell adhesion & migration
Cell culture
Cell growth
Cell migration
Cell viability
Chronic illnesses
Collagen
Cytotoxicity
Fibroblasts
Free radicals
Humidity
Nicotine
Oxidative stress
Signal transduction
Smoking
Toxicity
Wound healing
title Comparative effect of anthocyanin on proliferation and migration of human gingival fibroblasts in the absence or presence of nicotine
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