Fungicidal effect of combined nano TiO2 with erythrosine for mediated photodynamic therapy on Candida albicans: an in vitro study

• Published in: Lasers in dental science 2017-12, Vol.1 (2-4), p.101-106
• Main Authors: Teerakapong, Aroon, Damrongrungruang, Teerasak, Sattayut, Sajee, Morales, Noppawan Phumala, Sangpanya, Angkhana, Tanapoomchai, Manuswee
• Format: Article
• Language: English
• Subjects: Antifungal agents; Biofilms; Composite materials; Dentistry; Lasers; Medicine; Optical Devices; Optics; Original Article; Photodynamic therapy; Photonics; Reactive oxygen species; Titanium
• ISSN: 2367-2587; 2367-2587
• DOI: 10.1007/s41547-017-0014-z

Background and objective Candida albicans is an opportunistic pathogen that can cause oral candidiasis. Antifungal drugs have been used for treatment, but resistance to these drugs has emerged in recent years. Photodynamic therapy is one of the alternative treatments. The purpose of this study was to evaluate the killing of C. albicans biofilms in photodynamic therapy in vitro by erythrosine (Ery) gel on its own or in combination with nano-titanium dioxide (TiO 2 ) stimulated by blue light (BL). Methods Four test groups were studied, namely, Ery gel at 220 and 440 μM, in the presence or absence of 1% TiO 2 + BL, and the control group. After C. albicans biofilms were prepared, a photosensitizer was applied to them for 15 min. Test groups were then activated by BL with 15 J/cm 2 energy for 1 min, and the number of CFU/mL in log 10 was compared between the test and control groups. In addition, the generation of reactive oxygen species by the effective photosensitizer was tested by electron spin resonance spectrometer (ESR) using 2,2,6,6-tetramethyl-4-piperidone and 5,5-dimethyl-1-pyrroline N-oxide. Results C. albicans survival rates were different with statistical significance across all groups by the Kruskal-Wallis test ( p