Ethanol production via co-fermentation of C6 and C5 sugars from steam-pretreated sugarcane bagasse hydrolysates using non-GM yeasts Saccharomyces cerevisiae CAT-1 and Spathaspora hagerdaliae UFMG-CMY-303

• Published in: Biomass conversion and biorefinery 2024-03, Vol.14 (5), p.6359-6368
• Main Authors: Rech, Fernanda Roberta, Fontana, Roselei Claudete, Rosa, Carlos A., Baudel, Henrique Macedo, Ayub, Marco Antônio Záchia, Camassola, Marli, Dillon, Aldo J. A.
• Format: Article
• Language: English
• Subjects: Acetic acid; Bagasse; Bioreactors; Biotechnology; Cultivation; Energy; Ethanol; Fermentation; Hydrolysates; Hydrolysis; Original Article; Renewable and Green Energy; Sugar; Sugarcane; Superhigh frequencies
• ISSN: 2190-6815; 2190-6823
• DOI: 10.1007/s13399-022-02751-3

Co-cultivation involving Saccharomyces cerevisiae CAT-1 and native Spathaspora hagerdaliae UFMG-CM Y303 was used to produce ethanol from sugars obtained from steam-exploded sugarcane bagasse (SCB-SE) after enzymatic hydrolysis. The fermentation process was carried out under separate hydrolysis and fermentation (SHF) and simultaneous hydrolysis and fermentation (SSF). The use of co-cultivation enabled the conversion of both glucose and xylose present in the SCB-SE pretreated at 200 °C for 8 min to ethanol, reaching a production of 0.5 g of ethanol per g of SCB-SE in 5-L-volume bioreactors in SSF. It was found that acetic acid above a concentration of 2 g L −1 was harmful to ethanol production by the strain S. hagerdaliae UFMG-CM-Y303. The experimental results presented here contribute to the development of co-cultivation for the simultaneous conversion of C5 and C6 sugars for ethanol production.