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Generation of Induced Pluripotent Stem Cell Line iTAF15Xsk4 from Fibroblasts of a Patient with Microdeletion at Xq24
Differentiation of induced pluripotent stem cells (iPSCs) from patients and healthy donors allows in vitro study of genetic disorders. The authors have previously reported a clinical case of recurrent pregnancy loss in a patient with skewed X-chromosome inactivation in peripheral blood lymphocytes,...
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| Published in: | Russian journal of developmental biology 2023-12, Vol.54 (6), p.358-364 |
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| Main Authors: | , , , , , , , , , |
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| container_end_page | 364 |
| container_issue | 6 |
| container_start_page | 358 |
| container_title | Russian journal of developmental biology |
| container_volume | 54 |
| creator | Pristyazhnyuk, I. E. Meshcheryakov, N. I. Nikitina, T. V. Kashevarova, A. A. Fedotov, D. A. Tolmacheva, E. N. Minaycheva, L. I. Nazarenko, L. P. Lebedev, I. N. Menzorov, A. G. |
| description | Differentiation of induced pluripotent stem cells (iPSCs) from patients and healthy donors allows in vitro study of genetic disorders. The authors have previously reported a clinical case of recurrent pregnancy loss in a patient with skewed X-chromosome inactivation in peripheral blood lymphocytes, endometrium, and buccal epithelium. A 239 kb microdeletion at Xq24 that affected eight genes, including
UBE2A
, has been found. In this work, an iPSC line iTAF15Xsk4 was produced from the patient’s skin fibroblasts using nonintegrating episomal vectors. The iPSC line had a normal karyotype, expressed pluripotency markers, and expressed markers of all three germ layers upon differentiation in embryoid bodies. This cell line could be used for the
UBE2A
deficiency syndrome study. |
| doi_str_mv | 10.1134/S1062360423060073 |
| format | article |
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UBE2A
, has been found. In this work, an iPSC line iTAF15Xsk4 was produced from the patient’s skin fibroblasts using nonintegrating episomal vectors. The iPSC line had a normal karyotype, expressed pluripotency markers, and expressed markers of all three germ layers upon differentiation in embryoid bodies. This cell line could be used for the
UBE2A
deficiency syndrome study.</description><identifier>ISSN: 1062-3604</identifier><identifier>EISSN: 1608-3326</identifier><identifier>DOI: 10.1134/S1062360423060073</identifier><language>eng</language><publisher>Moscow: Pleiades Publishing</publisher><subject>Animal Anatomy ; Biomedical and Life Sciences ; Cell differentiation ; Collection of Pluripotent Stem Cell Lines ; Developmental Biology ; Endometrium ; Epithelium ; Fibroblasts ; Genetic disorders ; Histology ; Karyotypes ; Life Sciences ; Lymphocytes ; Morphology ; Peripheral blood ; Pluripotency ; Stem cell transplantation ; Stem cells ; X-chromosome inactivation</subject><ispartof>Russian journal of developmental biology, 2023-12, Vol.54 (6), p.358-364</ispartof><rights>Pleiades Publishing, Inc. 2023. ISSN 1062-3604, Russian Journal of Developmental Biology, 2023, Vol. 54, No. 6, pp. 358–364. © Pleiades Publishing, Inc., 2023. Russian Text © The Author(s), 2023, published in Ontogenez, 2023, Vol. 54, No. 6, pp. 397–404.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c198t-e5ee971dc95737f6a497c5ce9279be7e6ac2d9dbdaf5480253624cd4cc6bbae3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Pristyazhnyuk, I. E.</creatorcontrib><creatorcontrib>Meshcheryakov, N. I.</creatorcontrib><creatorcontrib>Nikitina, T. V.</creatorcontrib><creatorcontrib>Kashevarova, A. A.</creatorcontrib><creatorcontrib>Fedotov, D. A.</creatorcontrib><creatorcontrib>Tolmacheva, E. N.</creatorcontrib><creatorcontrib>Minaycheva, L. I.</creatorcontrib><creatorcontrib>Nazarenko, L. P.</creatorcontrib><creatorcontrib>Lebedev, I. N.</creatorcontrib><creatorcontrib>Menzorov, A. G.</creatorcontrib><title>Generation of Induced Pluripotent Stem Cell Line iTAF15Xsk4 from Fibroblasts of a Patient with Microdeletion at Xq24</title><title>Russian journal of developmental biology</title><addtitle>Russ J Dev Biol</addtitle><description>Differentiation of induced pluripotent stem cells (iPSCs) from patients and healthy donors allows in vitro study of genetic disorders. The authors have previously reported a clinical case of recurrent pregnancy loss in a patient with skewed X-chromosome inactivation in peripheral blood lymphocytes, endometrium, and buccal epithelium. A 239 kb microdeletion at Xq24 that affected eight genes, including
UBE2A
, has been found. In this work, an iPSC line iTAF15Xsk4 was produced from the patient’s skin fibroblasts using nonintegrating episomal vectors. The iPSC line had a normal karyotype, expressed pluripotency markers, and expressed markers of all three germ layers upon differentiation in embryoid bodies. This cell line could be used for the
UBE2A
deficiency syndrome study.</description><subject>Animal Anatomy</subject><subject>Biomedical and Life Sciences</subject><subject>Cell differentiation</subject><subject>Collection of Pluripotent Stem Cell Lines</subject><subject>Developmental Biology</subject><subject>Endometrium</subject><subject>Epithelium</subject><subject>Fibroblasts</subject><subject>Genetic disorders</subject><subject>Histology</subject><subject>Karyotypes</subject><subject>Life Sciences</subject><subject>Lymphocytes</subject><subject>Morphology</subject><subject>Peripheral blood</subject><subject>Pluripotency</subject><subject>Stem cell transplantation</subject><subject>Stem cells</subject><subject>X-chromosome inactivation</subject><issn>1062-3604</issn><issn>1608-3326</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNp1kE9LAzEQxYMoWKsfwFvA82r-bbY5lmJroWKhPfS2ZLOzmrrdtEmK-O3NWsGDeJqB-b33hofQLSX3lHLxsKJEMi6JYJxIQgp-hgZUklHGOZPnaU_nrL9foqsQtoRQkrgBijPowOtoXYddg-ddfTRQ42V79HbvInQRryLs8ATaFi9sB9iux1Oab8K7wI13Ozy1lXdVq0MMvYPGy-TW6z5sfMPP1nhXQwvfCTrizYGJa3TR6DbAzc8covX0cT15yhYvs_lkvMgMVaOYQQ6gCloblRe8aKQWqjC5AcUKVUEBUhtWq7qqdZOLEWE5l0yYWhgjq0oDH6K7k-3eu8MRQiy37ui7lFgyJRgjeYpJFD1R6dEQPDTl3tud9p8lJWXfbfmn26RhJ01IbPcK_tf5f9EXOlF7Dw</recordid><startdate>20231201</startdate><enddate>20231201</enddate><creator>Pristyazhnyuk, I. 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E.</creatorcontrib><creatorcontrib>Meshcheryakov, N. I.</creatorcontrib><creatorcontrib>Nikitina, T. V.</creatorcontrib><creatorcontrib>Kashevarova, A. A.</creatorcontrib><creatorcontrib>Fedotov, D. A.</creatorcontrib><creatorcontrib>Tolmacheva, E. N.</creatorcontrib><creatorcontrib>Minaycheva, L. I.</creatorcontrib><creatorcontrib>Nazarenko, L. P.</creatorcontrib><creatorcontrib>Lebedev, I. N.</creatorcontrib><creatorcontrib>Menzorov, A. G.</creatorcontrib><collection>CrossRef</collection><jtitle>Russian journal of developmental biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pristyazhnyuk, I. E.</au><au>Meshcheryakov, N. I.</au><au>Nikitina, T. V.</au><au>Kashevarova, A. A.</au><au>Fedotov, D. A.</au><au>Tolmacheva, E. N.</au><au>Minaycheva, L. I.</au><au>Nazarenko, L. P.</au><au>Lebedev, I. N.</au><au>Menzorov, A. 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UBE2A
, has been found. In this work, an iPSC line iTAF15Xsk4 was produced from the patient’s skin fibroblasts using nonintegrating episomal vectors. The iPSC line had a normal karyotype, expressed pluripotency markers, and expressed markers of all three germ layers upon differentiation in embryoid bodies. This cell line could be used for the
UBE2A
deficiency syndrome study.</abstract><cop>Moscow</cop><pub>Pleiades Publishing</pub><doi>10.1134/S1062360423060073</doi><tpages>7</tpages></addata></record> |
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| language | eng |
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| subjects | Animal Anatomy Biomedical and Life Sciences Cell differentiation Collection of Pluripotent Stem Cell Lines Developmental Biology Endometrium Epithelium Fibroblasts Genetic disorders Histology Karyotypes Life Sciences Lymphocytes Morphology Peripheral blood Pluripotency Stem cell transplantation Stem cells X-chromosome inactivation |
| title | Generation of Induced Pluripotent Stem Cell Line iTAF15Xsk4 from Fibroblasts of a Patient with Microdeletion at Xq24 |
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