The Proteome of Extracellular Membrane Vesicles from Bacillus pumilus 3-19

—Production of extracellular membrane vesicles plays an important role in communication in bacterial populations and in bacteria–host interactions. Vesicles as carriers of various regulatory and signaling molecules may be potentially used as disease biomarkers and promising therapeutic agents, inclu...

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Bibliographic Details
Published in:Molecular biology (New York) 2024-02, Vol.58 (1), p.157-164
Main Authors: Kurdy, W., Zelenikhin, P. V., Yakovleva, G. Yu, Sinyagina, M. N., Kolpakov, A. I., Ilinskaya, O. N.
Format: Article
Language:English
Subjects:
Amyloid
Antibiotic resistance
Bacillus pumilus
Biochemistry
Biomedical and Life Sciences
Cell culture
Flagella
Genomes
Gram-negative bacteria
Gram-positive bacteria
Human Genetics
Life Sciences
Membrane vesicles
Phosphate
Proteins
Proteomes
Proteomics
Ribonuclease
Signal peptides
Streptomycin
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Summary:—Production of extracellular membrane vesicles plays an important role in communication in bacterial populations and in bacteria–host interactions. Vesicles as carriers of various regulatory and signaling molecules may be potentially used as disease biomarkers and promising therapeutic agents, including vaccine preparations. The composition of membrane vesicles has been deciphered for a limited number of Gram-negative and Gram-positive bacteria. In this work, for the first time, extracellular membrane vesicles of a streptomycin-resistant strain Bacillus pumilus 3-19, a producer of extracellular guanyl-preferring ribonuclease binase, are isolated, visualized, and characterized by their genome and proteome composition. It has been established that there is no genetic material in the vesicles and the spectrum of the proteins differs depending on the phosphate content in the culture medium of the strain. Vesicles from a phosphate-deficient medium carry 49 unique proteins in comparison with 101 from a medium with the high phosphate content. The two types of vesicles had 140 mutual proteins. Flagellar proteins, RNase J, which is the main enzyme of RNA degradosomes, phosphatases, peptidases, iron transporters, signal peptides, were identified in vesicles. Antibiotic resistance proteins and amyloid-like proteins whose genes are present in B. pumilus 3-19 cells are absent. Phosphate deficiency-induced binase was found only in vesicles from a phosphate-deficient medium.
ISSN:0026-8933
1608-3245
DOI:10.1134/S0026893324010059