Development of a novel biosensor for the ultra-sensitive detection of the endocrine disruptor Bisphenol A and detection of Bisphenol A leakage from some plastic containers

• Published in: Journal of the Iranian Chemical Society 2024-03, Vol.21 (3), p.767-779
• Main Authors: Ergezer, E. E., Bodur, O. C., Özkan, E. Hasanoğlu, Sarı, N., Arslan, F.
• Format: Article
• Language: English
• Subjects: Analytical Chemistry; Biochemistry; Biosensors; Bisphenol A; Carbon; Chemistry; Chemistry and Materials Science; Crosslinking; Electrodes; Endocrine disruptors; Glutaraldehyde; Inorganic Chemistry; Leakage; Organic Chemistry; Physical Chemistry; Quinones; Reproducibility; Shelf life; Storage containers; Tyrosinase
• ISSN: 1735-207X; 1735-2428
• DOI: 10.1007/s13738-023-02959-8

In this study, a new biosensor was designed to the determination of Bisphenol A (BPA), an endocrine disruptor. First, poly(amidoamine) salicylidenimine Pd(II)-loaded PAMAM dendrimer was synthesized and characterized. Then, a modified carbon paste electrode was prepared with this Pd(II)-loaded PAMAM dendrimer. The biosensor was prepared by immobilizing the tyrosinase enzyme on the modified carbon paste electrode surface by the cross-linking method in the presence of glutaraldehyde. BPA determination was made by measuring the cathodic currents of the quinone compound formed due to this enzymatic reaction at − 0.10 V. In order to determine the best working conditions of the prepared BPA biosensor, linear operating range, polymer amount, glutaraldehyde amount, pH, temperature, BPA concentration effect, reproducibility, and shelf-life studies were performed. The prepared biosensor with a wide operating range (0.001–0.05 μM and 0.1–1.0 μM, ( R 2  = 0.9530 and R 2  = 0.9765)), a very low detection limit (0.001 µM) and good reproducibility was prepared (relative standard deviation of 9.43% after 18 measurements). The effects of species that may interfere with BPA determination on real samples were investigated. The prepared biosensor was used to detect BPA leakage from plastic food storage containers and plastic water bottles. Graphical abstract