Atomic Structure of a DNA‐Stabilized Ag11 Nanocluster with Four Valence Electrons

The combination of mass spectrometry and single crystal X‐ray diffraction of HPLC‐purified DNA‐stabilized silver nanoclusters (DNA‐AgNCs) is a powerful tool to determine the charge and structure of the encapsulated AgNC. Such information is not only relevant to design new DNA‐AgNCs with tailored pro...

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Bibliographic Details
Published in:Advanced optical materials 2024-03, Vol.12 (7), p.n/a
Main Authors: Rück, Vanessa, Neacșu, Vlad A., Liisberg, Mikkel B., Mollerup, Christian B., Ju, Park Hee, Vosch, Tom, Kondo, Jiro, Cerretani, Cecilia
Format: Article
Language:English
Subjects:
Atomic structure
Conjugation
Crystallization
Electronic structure
Electrons
fluorescence
HPLC purification
Mass spectrometry
Nanoclusters
silver nanoclusters
Single crystals
X-ray diffraction
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Summary:The combination of mass spectrometry and single crystal X‐ray diffraction of HPLC‐purified DNA‐stabilized silver nanoclusters (DNA‐AgNCs) is a powerful tool to determine the charge and structure of the encapsulated AgNC. Such information is not only relevant to design new DNA‐AgNCs with tailored properties, but it is also important for bio‐conjugation experiments and is essential for electronic structure calculations. Here, the efforts to determine the structure of a HPLC‐purified green emissive DNA‐AgNC are presented. Unfortunately, the original DNA‐AgNC, known to have four valence electrons, could not be crystallized. By modifying the stabilizing DNA sequence, while maintaining the original spectroscopic properties, several mutants could be successfully crystallized, and for one of them, single crystal X‐ray diffraction data provided insight into the silver positions. While the DNA conformation is not resolved, the described approach provides valuable insight into the class of green and dual emissive DNA‐AgNCs with four valence electrons. These results constitute a roadmap on how to improve crystallization and crystal quality for X‐ray diffraction measurements. A roadmap for determining the atomic structure of a DNA‐stabilized 4‐valence electron [Ag11]7+ nanocluster is presented. The combination of single crystal X‐ray diffraction and mass spectrometry allows for the first structural glimpse of this recurring green/dual emitter.
ISSN:2195-1071
2195-1071
DOI:10.1002/adom.202301928