Extracellular Matrix Alterations in Human Soleus Muscle under Conditions of Chronic Disorder of Consciousness

Critical illness myopathy (CIM) is a primary myopathy that develops in critically ill patients. Histological features of CIM include a general reduction in muscle fiber cross-sectional area and a predominant loss of the motor protein myosin in the absence of inflammatory infiltrates but with detecta...

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Bibliographic Details
Published in:Journal of evolutionary biochemistry and physiology 2024, Vol.60 (1), p.432-442
Main Authors: Zaripova, K. A., Tyganov, S. A., Turtikova, O. V., Skiteva, E. N., Zabrodskaya, Y. M., Kondratiev, S. A., Kondratieva, E. A., Kondratiev, A. N., Shenkman, B. S.
Format: Article
Language:English
Subjects:
Animal Physiology
Biochemistry
Biomedical and Life Sciences
Biopsy
Collagen
Collagen (type I)
Consciousness
Evolutionary Biology
Experimental Papers
Extracellular matrix
Fibronectin
Fibrosis
Inflammation
Life Sciences
Medical research
mRNA
Myopathy
Myosin
Neurosurgery
Patients
Skeletal muscle
Soleus muscle
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Summary:Critical illness myopathy (CIM) is a primary myopathy that develops in critically ill patients. Histological features of CIM include a general reduction in muscle fiber cross-sectional area and a predominant loss of the motor protein myosin in the absence of inflammatory infiltrates but with detectable cytokine activation. This study was aimed to examine the state of the soleus muscle extracellular matrix in patients with CIM caused by chronic disorders of consciousness. Incisional needle biopsies of the soleus muscle were taken from 6 patients with a chronic (≥ 2 months) disorder of consciousness, undergoing treatment at the Polenov Neurosurgical Institute (Almazov National Medical Research Center, St. Petersburg), and healthy men (control). Histological staining of soleus muscle sections in patients with CIM revealed a significantly increased collagen area that exceeded control values by 82%. Type I, III and VIa collagen mRNA levels, along with type I and III collagen content, were increased. No changes were found in fibronectin and extracellular tissue growth factor mRNA levels. However, integrin α7 mRNA levels were elevated. Our results indicate significant skeletal muscle fibrosis in CIM, requiring further studies on the signaling pathways that regulate this process.
ISSN:0022-0930
1608-3202
DOI:10.1134/S0022093024010332