Autologous Platelets-Rich Plasma Gel Promotes Neo-Vascularization and Re-Epithelization in Cutaneous Wound Healing in Rescued Horses

ABSTRACT Autologous platelet-rich plasma (PRP) gel has been suggested for wound repair. However, there is insufficient evidence to support its usage in animals with cutaneous wounds. Therefore, this study aimed to evaluate the effect of PRP gel on cutaneous wound healing in rescued horses. Twelve ho...

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Published in:Pakistan journal of zoology 2024-04, Vol.56 (2), p.579
Main Authors: Sajjad, Muhammad Talha, Akbar, Hamid, Khan, Muhammad Arif, Mushtaq, Muhammad Hassan, Bokhari, Shehla Gul, Hayat, Muhammad Abid, Mustafa, Ghulam
Format: Article
Language:English
Subjects:
Angiogenesis
Animals
Blood platelets
Care and treatment
Color
Cruelty to animals
Cytokeratin
Cytokines
Diagnosis, Ultrasonic
Doppler effect
Fibroblasts
Growth factors
Health aspects
Horses
Physiological aspects
Plasma
Platelet-rich plasma
Platelets
Staining
Ultrasonic imaging
Vascularization
Wound healing
Wounds and injuries
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container_issue 2
container_start_page 579
container_title Pakistan journal of zoology
container_volume 56
creator Sajjad, Muhammad Talha
Akbar, Hamid
Khan, Muhammad Arif
Mushtaq, Muhammad Hassan
Bokhari, Shehla Gul
Hayat, Muhammad Abid
Mustafa, Ghulam
description ABSTRACT Autologous platelet-rich plasma (PRP) gel has been suggested for wound repair. However, there is insufficient evidence to support its usage in animals with cutaneous wounds. Therefore, this study aimed to evaluate the effect of PRP gel on cutaneous wound healing in rescued horses. Twelve horses were divided into two groups: group A (PRP-treated) and group B (control). The group A (n=6) animals were treated with autologous PRP gel, while the group B (n=6) animals were treated with sterile saline. Full-thickness (3 x 3 cm2) skin wounds were selected in each horse on either left or right region of back. Neovascularization was assessed on days 14 and 28 by color Doppler ultrasonography and cytokeratin staining. Wound re-epithelialization and the number of fibroblasts were assessed by cytokeratin staining. All data were statistically analyzed. We observed that PRP-wounds had highly significantly (P
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However, there is insufficient evidence to support its usage in animals with cutaneous wounds. Therefore, this study aimed to evaluate the effect of PRP gel on cutaneous wound healing in rescued horses. Twelve horses were divided into two groups: group A (PRP-treated) and group B (control). The group A (n=6) animals were treated with autologous PRP gel, while the group B (n=6) animals were treated with sterile saline. Full-thickness (3 x 3 cm2) skin wounds were selected in each horse on either left or right region of back. Neovascularization was assessed on days 14 and 28 by color Doppler ultrasonography and cytokeratin staining. Wound re-epithelialization and the number of fibroblasts were assessed by cytokeratin staining. All data were statistically analyzed. We observed that PRP-wounds had highly significantly (P&lt;0.01) increased levels of neovascularization on days 14 and 28 than control wounds. PRP-wounds had highly significant (P&lt;0.01) increases in re-epithelialization levels and fibroblast numbers at days 14 and 28 than control wounds. In conclusion, PRP-treated wounds accelerated cutaneous wound healing by fostering re-epithelialization and neovascularization in rescued horses than control wounds. Therefore, this study suggests the use of autologous platelet-rich plasma gel to treat cutaneous wounds in rescued horses, which is a safe and effective method. The color Doppler ultrasonography can be used to assess neovascularization during wound healing.</description><identifier>ISSN: 0030-9923</identifier><identifier>EISSN: 0030-9923</identifier><identifier>DOI: 10.17582/journal.pjz/20220717180718</identifier><language>eng</language><publisher>Lahore: Knowledge Bylanes</publisher><subject>Angiogenesis ; Animals ; Blood platelets ; Care and treatment ; Color ; Cruelty to animals ; Cytokeratin ; Cytokines ; Diagnosis, Ultrasonic ; Doppler effect ; Fibroblasts ; Growth factors ; Health aspects ; Horses ; Physiological aspects ; Plasma ; Platelet-rich plasma ; Platelets ; Staining ; Ultrasonic imaging ; Vascularization ; Wound healing ; Wounds and injuries</subject><ispartof>Pakistan journal of zoology, 2024-04, Vol.56 (2), p.579</ispartof><rights>COPYRIGHT 2024 Knowledge Bylanes</rights><rights>(c)2024 Pakistan Journal of Zoology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27922,27923</link.rule.ids></links><search><creatorcontrib>Sajjad, Muhammad Talha</creatorcontrib><creatorcontrib>Akbar, Hamid</creatorcontrib><creatorcontrib>Khan, Muhammad Arif</creatorcontrib><creatorcontrib>Mushtaq, Muhammad Hassan</creatorcontrib><creatorcontrib>Bokhari, Shehla Gul</creatorcontrib><creatorcontrib>Hayat, Muhammad Abid</creatorcontrib><creatorcontrib>Mustafa, Ghulam</creatorcontrib><title>Autologous Platelets-Rich Plasma Gel Promotes Neo-Vascularization and Re-Epithelization in Cutaneous Wound Healing in Rescued Horses</title><title>Pakistan journal of zoology</title><description>ABSTRACT Autologous platelet-rich plasma (PRP) gel has been suggested for wound repair. However, there is insufficient evidence to support its usage in animals with cutaneous wounds. Therefore, this study aimed to evaluate the effect of PRP gel on cutaneous wound healing in rescued horses. Twelve horses were divided into two groups: group A (PRP-treated) and group B (control). The group A (n=6) animals were treated with autologous PRP gel, while the group B (n=6) animals were treated with sterile saline. Full-thickness (3 x 3 cm2) skin wounds were selected in each horse on either left or right region of back. Neovascularization was assessed on days 14 and 28 by color Doppler ultrasonography and cytokeratin staining. Wound re-epithelialization and the number of fibroblasts were assessed by cytokeratin staining. All data were statistically analyzed. We observed that PRP-wounds had highly significantly (P&lt;0.01) increased levels of neovascularization on days 14 and 28 than control wounds. 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ispartof Pakistan journal of zoology, 2024-04, Vol.56 (2), p.579
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0030-9923
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source Nexis UK
subjects Angiogenesis
Animals
Blood platelets
Care and treatment
Color
Cruelty to animals
Cytokeratin
Cytokines
Diagnosis, Ultrasonic
Doppler effect
Fibroblasts
Growth factors
Health aspects
Horses
Physiological aspects
Plasma
Platelet-rich plasma
Platelets
Staining
Ultrasonic imaging
Vascularization
Wound healing
Wounds and injuries
title Autologous Platelets-Rich Plasma Gel Promotes Neo-Vascularization and Re-Epithelization in Cutaneous Wound Healing in Rescued Horses
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