Application of Flow Cytometry for Viability Assay of Mutants for Translation Termination Factors in the Yeast Saccharomyces cerevisiae

Nonsense mutations in the essential SUP45 and SUP35 genes encoding translation termination factors affect the viability of Saccharomyces cerevisiae cells. Flow cytometry revealed that the viability of the mutants was 3.5‒4 times lower compared to the wild-type strains. Moreover, the mutants were fou...

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Bibliographic Details
Published in:Microbiology (New York) 2024-04, Vol.93 (2), p.236-239
Main Authors: Efremova, E. P., Zemlyanko, O. M., Zhouravleva, G. A.
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Flow cytometry
Life Sciences
Medical Microbiology
Microbiology
Mutants
Saccharomyces cerevisiae
Short Communications
Termination factors
Translation termination
ultrasonic treatment
Viability
viability assays
yeasts
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Summary:Nonsense mutations in the essential SUP45 and SUP35 genes encoding translation termination factors affect the viability of Saccharomyces cerevisiae cells. Flow cytometry revealed that the viability of the mutants was 3.5‒4 times lower compared to the wild-type strains. Moreover, the mutants were found to have higher sensitivity to ultrasonic treatment.
ISSN:0026-2617
1608-3237
DOI:10.1134/S0026261723604244