Metagenomic Analysis of Bottom Sediments of the Karst Meromictic Lake Black Kichier Revealed Abundant Unculturable Thermoplasmatota

Investigations into the microbial community of bottom sediments in the karst Lake Black Kichier were conducted. These sediments exhibited elevated levels of sulfide, dissolved methane, and organic matter. Direct radiotracer experiments revealed substantial rates of microbial processes involved in th...

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Bibliographic Details
Published in:Microbiology (New York) 2024-04, Vol.93 (2), p.128-133
Main Authors: Kadnikov, V. V., Savvichev, A. S., Rusanov, I. I., Beletskii, A. V., Pimenov, N. V.
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Carbon cycle
Life Sciences
Medical Microbiology
Meromictic lakes
Metagenomics
Microbiology
Microbiomes
Organic matter
Sediments
Short Communications
Thermoplasmata
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Summary:Investigations into the microbial community of bottom sediments in the karst Lake Black Kichier were conducted. These sediments exhibited elevated levels of sulfide, dissolved methane, and organic matter. Direct radiotracer experiments revealed substantial rates of microbial processes involved in the decomposition of organic matter. Uncultivated archaea belonging to the phylum Thermoplasmatota were identified within the microbial community. Metagenomic analysis unveiled representatives from five orders: Methanomassiliicoccales , Thermoprofundales (formerly known as Marine Benthic Group D and DHVEG-1), DTX01, SG8-5, and Candidatus Gimiplasmatales (formerly UBA10834). These archaea were previously believed to occur exclusively in deep marine sediments characterized by extreme organic matter scarcity. This discovery reshapes our understanding of the role played by Thermoplasmatota archaea, spanning five orders, in the degradation segment of the carbon cycle.
ISSN:0026-2617
1608-3237
DOI:10.1134/S0026261723604359