Comparison of molecular markers to distinguish genotypes of Eurasian watermilfoil, northern watermilfoil, and their hybrids

In this study, we specifically tested the hypothesis of whether individuals with the same MMGs represented ramets of the same genets (i.e., clones, or individuals that share common ancestry through asexual reproduction) versus having the same MMG by chance through sexual reproduction. [...]we chose...

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Bibliographic Details
Published in:Journal of aquatic plant management 2020-01, Vol.58 (1), p.61-66
Main Authors: Pashnick, Jeff, Thum, Ryan A
Format: Article
Language:English
Subjects:
Alleles
Amplified fragment length polymorphism
Analysis
Aquatic plants
Asexual reproduction
Clones
Data analysis
Data collection
Genetic aspects
Genetic diversity
Genets
Genomes
Genotype
Genotype & phenotype
Genotypes
Heterozygotes
Hybrids
Laboratories
Lakes
Microsatellites
Ramets
Reproduction
Sexual reproduction
Software
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Summary:In this study, we specifically tested the hypothesis of whether individuals with the same MMGs represented ramets of the same genets (i.e., clones, or individuals that share common ancestry through asexual reproduction) versus having the same MMG by chance through sexual reproduction. [...]we chose individuals for this study by identifying individuals that shared the same MMG within and/or among lakes in the Michigan and Minnesota data sets described above. EWM, NWM, and hybrids are hexapioid, and therefore their exact genotypes cannot be determined because of potential differences in allele dosage in heterozygotes. [...]we treated microsatellite data as dominant (presence or absence of all possible alleles at each locus) (see also Wu et al. 2015a,b). AFLP data collection and analysis AFLP data were collected on 192 individuals (Table 1) using methods described in Zuellig and Thum (2012). To visualize relatedness among genotypes, and confirm that individuals belonging to the same MMG were most closely related to each other as opposed to other MMGs, we ran a principal coordinates analysis (PCoA) using GenAlEx version 6.503 (Peakall and Smouse 2006). ddRAD data collection and analysis We also used a next-generation GBS approach, ddRAD sequencing (Peterson et al. 2012), to genotype our 192 individuals.
ISSN:0146-6623