Flow Behavior Characterization of DNA Molecules in Passive Nanofluidic Devices

This work aims to develop a method for analyzing the dynamic flow of single DNA molecules through nanochannels in fluidic chip which work passively. For this purpose, a two‐laser system was used, which is coupled into a fluorescence microscope and can detect labeled DNA molecules using a single phot...

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Bibliographic Details
Published in:IEEJ transactions on electrical and electronic engineering 2024-05, Vol.19 (5), p.840-844
Main Authors: Esmek, Franziska M., Grzybeck, Phil, Nasri, Rukan, Tiwari, Sadhana, Fernandez‐Cuesta, Irene
Format: Article
Language:English
Subjects:
DNA flow in nanochannels
DNA speed and stretching
flow characterization
Fluidics
microfluidics
Nanochannels
nanofluidics
Nanofluids
passive flow devices
Photon counters
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Summary:This work aims to develop a method for analyzing the dynamic flow of single DNA molecules through nanochannels in fluidic chip which work passively. For this purpose, a two‐laser system was used, which is coupled into a fluorescence microscope and can detect labeled DNA molecules using a single photon counter for signal read‐out in real‐time. The two laser spots are focused at different points of the nanochannel, and can be used to obtain the speed and molecular extension of the DNA. For example, a velocity of 1.82 μm/ms and a spatial extension of 0.9 μm were determined for pUC18/19 DNA (2686 bp) in a nanochannel with a width and depth of 200 nm × 100 nm and a length of 10 μm. © 2024 Institute of Electrical Engineer of Japan and Wiley Periodicals LLC.
ISSN:1931-4973
1931-4981
DOI:10.1002/tee.24059