Genome-Wide Analysis of Glutathione S-Transferase Gene Family in P. vulgaris Under Drought and Salinity Stress

• Published in: Plant molecular biology reporter 2024-03, Vol.42 (1), p.57-76
• Main Authors: Muslu, Selman, Kasapoğlu, Ayşe Gül, Güneş, Ebru, Aygören, Ahmed Sidar, Yiğider, Esma, İlhan, Emre, Aydın, Murat
• Format: Article
• Language: English
• Subjects: Amino acids; Beans; Bioinformatics; Biomedical and Life Sciences; Cultivars; Drought; Exons; French beans; Gene expression; Genes; Genomes; Glutathione; Glutathione transferase; Heavy metals; Isoelectric points; Life Sciences; Melatonin; Metabolomics; Phaseolus vulgaris; Phylogeny; Plant Breeding/Biotechnology; Plant Sciences; Plant tissues; Proteins; Proteomics; Reactive oxygen species; Salinity; Salinity effects; Stresses
• ISSN: 0735-9640; 1572-9818
• DOI: 10.1007/s11105-023-01400-x

The enzymes known as glutathione S-transferases (GST), which are present in many evolved organisms, are essential for the defense against reactive oxygen species. GSTs have a role in the development of defenses against biotic and abiotic challenges, especially defending plants from various stresses such as drought, salinity, and heavy metal. This study uses a genome-wide investigation of the GST gene family in Phaseolus vulgaris to pinpoint several distinctive traits. 55 Pv-GST proteins have been identified in P. vulgaris . The molecular weights of these proteins range from 15.02 kDa to 47.99 kDa; the range of amino acid numbers 132 to 420 and the range of theoretical isoelectric points 5.03 to 9.61 were identified. Pv-GST genes are estimated to have at least 2 and a maximum of 10 exons, with an average of 4 exons. Phylogenetic analysis was performed with GST proteins from Arabidopsis thaliana , Glycine max , and Phaseolus vulgaris species, and subfamilies of these GSTs were identified. Using RNAseq data, the expression profiles of Pv-GST genes in leaf tissue of common bean during drought and salinity stress were identified. Using the obtained sequence data, primers for qRT-PCR were designed. Changes in the expression profiles of GST genes caused by salt and drought stress and melatonin treatments in two different common bean cultivars were determined by qRT-PCR experiments. Under drought and salt stress, the expression levels of GSTs decreased, while melatonin treatment with few exceptions increased these expression levels. As a result of this study, it was observed that Pv-GST genes may play a role in the growth and development of bean and may be involved in the response to abiotic stresses. Moreover, the results of this study will provide a basis for functional gene research and the expression profiles and qRT-PCR results under different stress conditions in common bean will provide a valuable contribution to the understanding of the functions of the GST gene family.