Iso‐E‐Codelock: A Rebuilding‐free Electrochemical Chip with a Customizable Decoding Probe for Real‐Time and Portable Pathogen Diagnostics

In order to realize portable pathogen diagnostics with easier quantitation, digitization and integration, we develop a ready‐to‐use electrochemical sensing strategy (Iso‐E‐Codelock) for real‐time detection of isothermal nucleic acid amplification. Bridged by a branched DNA as codelock, the isotherma...

Full description

Saved in:
Bibliographic Details
Published in:Angewandte Chemie 2024-05, Vol.136 (19), p.n/a
Main Authors: Liu, Yichen, Tang, Yidan, Bao, Yin, Cai, Kaiwei, Lu, Baiyang, Zhao, Rujian, Yu, Chunxu, Du, Yan, Li, Bingling
Format: Article
Language:English
Subjects:
African swine fever
Amplification
disposable microchip
DNA probes
Electrochemistry
Integrated circuits
isothermal amplification
Nucleic acids
pathogen diagnostics
Pathogens
Portability
portable instrument
real-time electrochemical detection
Rebuilding
Severe acute respiratory syndrome coronavirus 2
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In order to realize portable pathogen diagnostics with easier quantitation, digitization and integration, we develop a ready‐to‐use electrochemical sensing strategy (Iso‐E‐Codelock) for real‐time detection of isothermal nucleic acid amplification. Bridged by a branched DNA as codelock, the isothermal amplicon is transduced into increased current of an electrochemical probe, holding multiple advantages of high sensitivity, high selectivity, signal‐on response, “zero” background and one‐pot operation. Through a self‐designed portable instrument (BioAlex PHE−T), the detection can be implemented on a multichannel microchip and output real‐time amplification curves just like an expensive commercial PCR machine. The microchip is a rebuilding‐free and disposable component. The branch codelock probe can be customized for different targets and designs. Such high performance and flexibility have been demonstrated utilizing four virus (SARS‐CoV‐2, African swine fever, FluA and FluB) genes as targets, and two branch (3‐way and 4‐way) DNAs as codelock probes. We develop an electrochemical sensing strategy (Iso‐E‐Codelock) and a ready‐to‐use multichannel instrument (BioAlex PHE−T) for portable isothermal amplification detection. Our method provides high sensitivity & selectivity, signal‐on response, “zero” background, one‐pot operation and real‐time signal collection.
ISSN:0044-8249
1521-3757
DOI:10.1002/ange.202400340