A four steps protocol for in vitro propagation of Eugenia uniflora L. (Myrtaceae)

Eugenia uniflora L. (Myrtaceae) is a key species in different forest formations with ecological, economic, and medicinal importance. This study aimed at establishing an in vitro culture procedure for the micropropagation of E. uniflora genotypes through organogenesis. Nodal segments were introduced...

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Bibliographic Details
Published in:Genetic resources and crop evolution 2024-06, Vol.71 (5), p.1813-1823
Main Authors: Franco, Lucas Barasuol, Hermann, Bruna Ronchi, Fritsche, Yohan, Stefenon, Valdir Marcos
Format: Article
Language:English
Subjects:
Agriculture
Biomedical and Life Sciences
Carotenoids
Chlorophyll
Culture media
Eugenia uniflora
Experiments
Flasks
Fruits
Gas exchange
Genotypes
Growth regulators
Immersion
Leaf area
Life Sciences
Mass production
Metabolites
Micropropagation
Myrtaceae
Organogenesis
Plant Genetics and Genomics
Plant growth
Plant Physiology
Plant Sciences
Plant Systematics/Taxonomy/Biogeography
Propagation
Research Article
Rooting
Seedlings
Seeds
Semisolids
Submerging
Sucrose
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Summary:Eugenia uniflora L. (Myrtaceae) is a key species in different forest formations with ecological, economic, and medicinal importance. This study aimed at establishing an in vitro culture procedure for the micropropagation of E. uniflora genotypes through organogenesis. Nodal segments were introduced in a semi-solid medium for organogenesis establishment and posteriorly cultivated in immersion systems for shoot development and rooting. Different culture media, plant growth regulator concentrations, and immersion systems were tested. The highest rate of shoot generation was obtained with 2.0–2.5 µM BAP + 0.5 µM of NAA. The measures related to rooting did not differ among the tested immersion systems, but the biomass accumulation and leaf area were significantly higher in the twin-flasks system. There were no significant differences in chlorophyll contents among the immersion systems. A significantly higher content of carotenoids was observed in the permanent immersion system with gas exchange. The protocol provided in this study may be helpful for the mass production of seedlings for commercial orchard implementation, as well as in genetic improvement programs for E. uniflora .
ISSN:0925-9864
1573-5109
DOI:10.1007/s10722-023-01741-2