Continuous fermentation using high cell density cell recycle system for L-lactic acid production

For complete utilization of high glucose at ∼100 g/L, a high cell density (HCD) continuous fermentation system was established using Lb. delbrueckii NCIM 2025 for the bioproduction of lactic acid (LA). An integrated membrane cell recycling system coupled with the continuous bioreactor, aided to achi...

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Bibliographic Details
Published in:Preparative biochemistry & biotechnology 2024-05, Vol.54 (5), p.668-679
Main Authors: Gupta, Vaishali, Odaneth, Annamma A., Lali, Arvind M.
Format: Article
Language:English
Subjects:
Acid production
Bioreactors
Cell density
cell recycle
continuous fermentation
Density
Dilution
Fermentation
Glucose
high cell density
Lactic acid
Productivity
Recycling systems
Sustainability
Wet cells
yield
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Summary:For complete utilization of high glucose at ∼100 g/L, a high cell density (HCD) continuous fermentation system was established using Lb. delbrueckii NCIM 2025 for the bioproduction of lactic acid (LA). An integrated membrane cell recycling system coupled with the continuous bioreactor, aided to achieve the highest 34.77 g/L h LA productivity and 0.94−0.98 g/g yield. ∼34 times higher productivity was observed (in comparison to batch fermentation conducted in this study), when the continuous operations were carried out at the maximum dilution rate and wet cell weight i.e. 0.36 h −1 and 230 g/L, respectively. These results show the potential of this method for large-scale lactic acid production because it not only produces high titers but also ensures that glucose is used effectively. The method's superior performance in comparison to earlier studies suggests it as an affordable and sustainable alternative for the production of LA.
ISSN:1082-6068
1532-2297
DOI:10.1080/10826068.2023.2268207