Continuous fermentation using high cell density cell recycle system for L-lactic acid production

• Published in: Preparative biochemistry & biotechnology 2024-05, Vol.54 (5), p.668-679
• Main Authors: Gupta, Vaishali, Odaneth, Annamma A., Lali, Arvind M.
• Format: Article
• Language: English
• Subjects: Acid production; Bioreactors; Cell density; cell recycle; continuous fermentation; Density; Dilution; Fermentation; Glucose; Glucose - metabolism; high cell density; Lactic acid; Lactic Acid - biosynthesis; Lactic Acid - metabolism; Lactobacillus delbrueckii - growth & development; Lactobacillus delbrueckii - metabolism; Productivity; Recycling systems; Sustainability; Wet cells; yield
• ISSN: 1082-6068; 1532-2297
• DOI: 10.1080/10826068.2023.2268207

For complete utilization of high glucose at ∼100 g/L, a high cell density (HCD) continuous fermentation system was established using Lb. delbrueckii NCIM 2025 for the bioproduction of lactic acid (LA). An integrated membrane cell recycling system coupled with the continuous bioreactor, aided to achieve the highest 34.77 g/L h LA productivity and 0.94−0.98 g/g yield. ∼34 times higher productivity was observed (in comparison to batch fermentation conducted in this study), when the continuous operations were carried out at the maximum dilution rate and wet cell weight i.e. 0.36 h −1 and 230 g/L, respectively. These results show the potential of this method for large-scale lactic acid production because it not only produces high titers but also ensures that glucose is used effectively. The method's superior performance in comparison to earlier studies suggests it as an affordable and sustainable alternative for the production of LA.