Assessment of the effects of 2,4-D-dichlorophenoxyacetic acid-based herbicide exposure in eggs and embryos of golden apple snails using OCT

This study aimed to evaluate the effects of herbicide 2, 4-D-dichlorophenoxy acetic acid on golden apple snail eggs and embryos. Additionally, the study assessed the applicability of optical coherence tomography (OCT), a non-invasive depth cross-sectional microscopic imaging technique, as a novel me...

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Bibliographic Details
Published in:Applied optics (2004) 2024-05, Vol.63 (13), p.3712
Main Authors: Thanomsit, Chutima, Khumsopha, Chaiprasarn, Saetiew, Jadsada, Meemon, Panomsak, Nanthanawat, Phochit, Saowakoon, Samnao
Format: Article
Language:English
Subjects:
2,4-Dichlorophenoxyacetic Acid - pharmacology
2,4-Dichlorophenoxyacetic Acid - toxicity
Acetic acid
Animals
Dichlorophenoxyacetic acid
Embryo, Nonmammalian - drug effects
Embryo, Nonmammalian - embryology
Exposure
Herbicides
Herbicides - pharmacology
Herbicides - toxicity
Imaging techniques
Morphology
Optical Coherence Tomography
Optical microscopy
Ovum - drug effects
Snails
Snails - drug effects
Snails - embryology
Tomography, Optical Coherence - methods
White light
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Summary:This study aimed to evaluate the effects of herbicide 2, 4-D-dichlorophenoxy acetic acid on golden apple snail eggs and embryos. Additionally, the study assessed the applicability of optical coherence tomography (OCT), a non-invasive depth cross-sectional microscopic imaging technique, as a novel method, to the best of our knowledge, for studying morphological changes in golden apple snail eggs and embryos, in comparison to the conventional approach of using white light microscopy. The study revealed that the herbicide 2,4-D-dichlorophenoxy acetic acid affected the hatchery rate and morphological changes of the eggs and embryos. The lethal concentration (LC50), representing the concentration of a substance that is expected to cause death in half of the population being studied, of the golden apple eggs and embryos increased with longer exposure time and higher concentrations. The estimated median effective concentration (EC50), which denotes the concentration producing the desired effect in 50% of the exposed golden apple embryos, exhibited a similar trend of change as the LC50. When compared to the microscopic study, it was observed that OCT could be employed to investigate morphological changes of golden apple snail eggs and embryos, enabling evaluation of alterations in both 2D and 3D structures.
ISSN:1559-128X
2155-3165
DOI:10.1364/AO.503470