Pharmaceuticals mesalazine estimation by spectrophotometric diazotization and coupling reaction with 4-chlororesorcinol

• Main Authors: Younus, Mays M., Omar, Khalida M.
• Format: Conference Proceeding
• Language: English
• Subjects: Absorptivity; Chemical reactions; Coupling; Hydrochloric acid; Pharmaceuticals; Reagents; Sodium hydroxide; Sodium nitrite; Spectrophotometry
• ISSN: 0094-243X; 1551-7616
• DOI: 10.1063/5.0210086

Our purpose of the current paper is to develop a soft, simple, accurate and highly sensitive spectrophotometric procedure for the quantity estimation of mesalazine for both in pure and pharmaceuticals form. The method relied on the reaction of mesalazin (MEZ) with equimolar concentration of in sodium nitrite in present of hydrochloric acid solution(3.0M), to form diazonium salt ion, then coupling it with 4-chlororesorcinol in medium of sodium hydroxide to yield an orange –red dye dissolves in water and stable, which exhibited high absorption at 485nm versus reagent blank under the optimum conditions. The linearity of the proposed method follows Beer’s law in the concentration rang of (4-140µg/20ml), i,e 0.2-7.0 ppm with an excellent determination,as well as coefficient R2 =0.9975, the apparent molar absorptivity is equal to 2.66x10 4 l.mol1.cm-1 and sandells sensitivity index 0.00574 µg .cm-2. The limit of detection (LOD) and quantification (LOQ) are equal to 0.01369 and 0.0456 µg/ml, respectively. Suggested procedure has an excellent recovery when is applied for the estimation of MEZ in available dosage forms (tablets), the method was free from excipients interference.