Biocompatible compositions based on chitosan and collagen with high strength characteristics

• Published in: Journal of polymer research 2024-05, Vol.31 (5), Article 138
• Main Authors: Apryatina, Kristina V., Bobrynina, Elizaveta A., Monina, Alina P., Semenycheva, Lyudmila L., Zaitsev, Sergey D., Cherkasova, Elena I., Smirnova, Larisa A.
• Format: Article
• Language: English
• Subjects: Adhesion; Biocompatibility; Block copolymers; Cell growth; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Chitosan; Collagen; Composition; Density; Fibroblasts; Industrial Chemistry/Chemical Engineering; Mechanical properties; Original Paper; Polymer Sciences; Tensile strength; Tissue engineering
• ISSN: 1022-9760; 1572-8935
• DOI: 10.1007/s10965-024-03984-8

Block copolymers based on fish collagen and chitosan were obtained by ultrasonic irradiation of a blend of homopolymers. The molecular weight of the block copolymer was 310 kDa. The tensile strength of the block copolymer film increases by more than 200% compared to the original chitosan and by 145% compared to films based on a mechanical blend of collagen and chitosan, and amounts to 123 MPa. The growth density of hTert-BJ5ta fibroblast cells on the surface of film samples was calculated. For chitosan, the cell growth density was 97,000 cells/cm 2 . Films made from mechanical blend were characterized by the lowest adhesion and cell proliferation - 35,000 cells/cm 2 . However, the block copolymer films had a significant cell growth density - 90,000 cells/cm 2 , which is close to the values of the original chitosan. The totality of the results obtained shows that compositions based on block copolymers are superior to films made from homopolymers and their blends in mechanical properties, adhesion and proliferation of fibroblast cells, which is important for their use in tissue engineering.