Antioxidant activity of bottlebrush copolymers and its self-assembly in mixed monolayers with cholesterol in the presence of cerium nitrate

• Published in: Journal of polymer research 2024-05, Vol.31 (5), Article 144
• Main Authors: Melnikova, Nina, Zamyshlyayeva, Olga, Kamorin, Denis, Kazantsev, Oleg, Simagin, Alexander, Sheferova, Alyona, Baten′kin, Maxim
• Format: Article
• Language: English
• Subjects: Antioxidants; Biological properties; Catalase; Cationic polymerization; Cerium compounds; Cerium nitrate; Cerium oxides; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Cholesterol; Compressibility; Copolymers; Drug delivery systems; Ethylene glycol; Industrial Chemistry/Chemical Engineering; Methacrylamide; Monolayers; Nitrates; Original Paper; Polymer Sciences; Polymers; Propylene; Self-assembly; Wettability
• ISSN: 1022-9760; 1572-8935
• DOI: 10.1007/s10965-024-03999-1

The non-toxic amphiphilic bottlebrush polymers capable of self-organization on various interfaces and including cerium ions in structural assemblies without changing their biological properties have great importance for medicine and pharmacy. In the work, we studied the self-assembly in mixed monolayers of two copolymers – methoxy[oligo(propylene glycol)] methacrylate and methoxy[oligo(ethylene glycol)] methacrylate (PK) and its analogue with N-[3-(dimethylamino)propyl]methacrylamide) units quaternized with methyl iodide (PK q ), and cholesterol on the aqueous subphase-air interface in the present of cerium compounds. The effect of the polymer-cerium composition on the oxidoruductase activity was investigated as well. The cationic polymer PK q was shown to exhibit higher specific activity of antioxidant enzymes than the non-ionic PK copolymer, increasing the activity of SOD by 20%, catalase – by 15%, GR – by 24%, G6PD – by 88%, and AlDH – by 10%. In the presence of cerium compounds (Ce(NO 3 ) 3 , NH 4 [Ce(NO 3 ) 3 ], CeO 2 nanoparticles) these effects increased significantly. Cholesterol in a monolayer of polymers contributed to an increase of compressibility modulus C s −1 under the influence of cerium ions in mixed monolayers by approximately two times compared to mixed Chol+polymers monolayers on water, and C s −1 became 640 mN/m for Chol+PK monolayers and 700 mN/m for Chol+PK q monolayers. AFM and wettability study suggest elastic structure of transferred mixed monolayers that is suitable for designing a drug delivery system.