Effect of incubation temperature after high hydrostatic pressure treatment of Escherichia coli on the detection of injured populations

Escherichia coli in phosphate-buffered saline was treated with high hydrostatic pressures (HHPs) of 400, 500, and 600 MPa at 25 °C for 10 min. The cell suspension was then plated on nonselective agar and incubated at 20, 25, 30, 35, 37, and 40 °C to study the effect of the incubation temperatures on...

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Bibliographic Details
Published in:High pressure research 2024-04, Vol.44 (2), p.105-115
Main Authors: Nakaura, Yoshiko, Sok, Claudia, Morimatsu, Kazuya, Yamamoto, Kazutaka
Format: Article
Language:English
Subjects:
Cold storage
detection
E coli
Escherichia coli
High hydrostatic pressure
Hydrostatic pressure
incubation
Injuries
injury
Populations
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Summary:Escherichia coli in phosphate-buffered saline was treated with high hydrostatic pressures (HHPs) of 400, 500, and 600 MPa at 25 °C for 10 min. The cell suspension was then plated on nonselective agar and incubated at 20, 25, 30, 35, 37, and 40 °C to study the effect of the incubation temperatures on the detection of HHP-injured populations. The number of detected cells was maximum when incubated at 25 °C for 72 h. Cold storage at 4 °C for 7 d after HHP-treatment improved the detection efficacies of incubations at 30-40 °C, implicating increased tolerance of HHP-injured cells against the mild heat. Combined incubation at 25 °C for more than 6 h with subsequent incubation at 35 °C shortened total incubation period from 72 h to 24 h while achieving the maximized colony number obtained by the incubation at 25 °C for 72 h.
ISSN:0895-7959
1477-2299
DOI:10.1080/08957959.2024.2336468