Gordonibacillus kamchatkensis gen. nov., sp. nov. from the Frozen Volcanic Ash

• Published in: Microbiology (New York) 2024-06, Vol.93 (3), p.269-281
• Main Authors: Kudryashova, E. B., Ariskina, E. V., Karlyshev, A. V., Potekhina, N. V., Shashkov, A. S., Suzina, N. E., Evtushenko, L. I.
• Format: Article
• Language: English
• Subjects: Biomedical and Life Sciences; Cardiolipin; Cell walls; Experimental Articles; Galactose; Genomes; Glycerol; Hydrolysates; Life Sciences; Lipids; Medical Microbiology; Menaquinones; Microbiology; New genera; New species; Nucleotide sequence; Paenibacillus; Paenibacillus polymyxa; Peptidoglycans; Phosphatidylglycerol; Phosphatidylinositol; rRNA 16S; Teichoic acids; Volcanic ash
• ISSN: 0026-2617; 1608-3237
• DOI: 10.1134/S0026261723603706

An aerobic, rod-shaped, non-motile, endospore-forming bacterium, strain V-9 T , was found in the frozen volcanic ash, Kamchatka peninsula, Russia. Phylogenies based on 16S rRNA gene and genome sequences demonstrated that strain V-9 T was affiliated with the family Paenibacillaceae and clustered outside the clade that includes the type species of this genus, Paenibacillus polymyxa, and closely related species. V-9 T showed the highest 16S rRNA gene sequences similarity to Paenibacillus chartarius CCUG 55240 T (96.7%). The similarity to type strains of other Paenibacillus species were below 94.7%, with 91.8% determined to Paenibacillus polymyxa IAM 13419 T . The AAI and POCP values between V-9 T and type species of the genera of Paenibacillaceae with available genomes, including Paenibacillus polymyxa, were below the thresholds for defining novel genera. The cell wall of new strain contained a lysine-based peptidoglycan and two teichoic acids, unsubstituted 1,3-poly(glycerol phosphate) and 1,3-poly(glycerol phosphate) substituted at the C(2) of glycerol by glucose. Acid hydrolysates of the cell wall included glucose, galactose and glycerol. Strain V-9 T contained menaquinone MK-7 as the sole isoprenoid quinone, anteiso-C 15:0 , anteiso-C 17:0 , iso- C 15:0 and iso-C 16:0 a as the dominant fatty acids, and phosphatidylglycerol, diphosphatidylglycerol, acylphosphatidylglycerol, and phosphatidylinositol as the major polar lipids. The DNA G+C content calculated from the whole genome sequence was 55 mol %. Based on the data obtained, a new genus and species, Gordonibacillus kamchatkensis gen. nov., sp. nov., is proposed with the type strain V-9 T (=VKM B-2647 T = DSM 26726 T ).