Biocompatible nano-bandage modified with silver nanoparticles based on herbal for burn treatment

• Published in: Polymer bulletin (Berlin, Germany) Germany), 2024-06, Vol.81 (9), p.8285-8314
• Main Authors: Karami, Rozhin, Moradipour, Pouran, Arkan, Elham, Zarghami, Reza, Rashidi, Khodabakhsh, Darvishi, Elahe
• Format: Article
• Language: English
• Subjects: Biocompatibility; Burns; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Chitosan; Complex Fluids and Microfluidics; Contact angle; Drug delivery systems; E coli; Electrospinning; Energy consumption; Fibroblasts; Gelatin; Gram-positive bacteria; Homeostasis; In vivo methods and tests; Injuries; Mechanical properties; Multilayers; Nanofibers; Nanoparticles; Organic Chemistry; Original Paper; Permeability; Physical Chemistry; Polyacrylonitrile; Polymer Sciences; Polymers; Polyvinyl alcohol; Silver; Skin diseases; Soft and Granular Matter; Staphylococcus infections; Surface charge; Water absorption; Wound healing
• ISSN: 0170-0839; 1436-2449
• DOI: 10.1007/s00289-023-05093-w

Electrospinning has garnered much attention for skin renewal, emphasizing skin’s pivotal role as a primary protective layer and the susceptibility to loss caused by burns. The research aimed to develop a multipurpose wound dressing that safeguard injuries and facilitates the renewal of dermal tissues. Two-layer nanofibers were prepared using polyvinyl alcohol–chitosan–gelatin/polyacrylonitrile (PVA–CS–Gel/PAN), containing mupirocin (Mu 3% w/w) in underlying layer and silver nanoparticles (AgNps) in the upper layer with varying concentrations. AgNps were synthesized from Capsella bursa-pastoris extract by the green method and characterized using XRD, SEM, FTIR, and UV techniques. Then, PVA–CS–Gel/PAN solutions with AgNps and Mu were electrospun into multilayer dressings. The effectiveness of the nanofibers was evaluated through in vitro and in vivo tests. The study examined the nanofibers containing spherical AgNps with an average diameter of 72.57 nm and a negative surface charge (− 12 mv). They had uniform and smooth surfaces with a diameter range of 476.31 to 926.04 nm. According to swelling and contact angle results, hydrophilicity of samples had a direct relation with water absorption. Controlled drug release within 72 h followed Higuchi or first-order profiles. MTT and antibacterial analyses indicated that optimized nanofibers (Mu/1% AgNps) had suitable biocompatibility and synergistic potential against Escherichia coli ( E. coli ) and Staphylococcus aureus ( S. aureus ) bacteria. Additionally, an in vivo test on rats with second-degree burns after 28 days demonstrated a 98.97% improvement in revival, outperforming its commercial counterparts. Consequently, the results position the designed composite nanofiber as a promising candidate for wound healing applications, aiding in enhancing skin regeneration.