Study of the Effect of AdipoRon on Caveolin 1 Gene Expression in Human Adipocytes

Background: AdipoRon is an agonist of adiponectin receptors, and this molecule has demonstrated great potential for the treatment of various diseases, including obesity. However, its relationship with caveolin-1 (CAV-1), an integral membrane protein that interacts with adiponectin receptors (AdipoR1...

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Bibliographic Details
Published in:Obesity (Silver Spring, Md.) Md.), 2023-11, Vol.31, p.78-79
Main Authors: Camargo, Doris Gomez, Garcia, Dacia Malambo, Barreto, Sindy Cabarca, Mendoza, Maria Fontalvo, Gomez-Alegria, Claudio, Cano-Perez, Eder
Format: Article
Language:English
Subjects:
Adipocytes
Body fat
Diabetes
Gene expression
Obesity
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Summary:Background: AdipoRon is an agonist of adiponectin receptors, and this molecule has demonstrated great potential for the treatment of various diseases, including obesity. However, its relationship with caveolin-1 (CAV-1), an integral membrane protein that interacts with adiponectin receptors (AdipoR1/R2), remains unknown. Therefore, the objective of this study was to analyze the changes in CAV-1 gene expression associated with the exposure of human adipocytes to AdipoRon. Methods: An experimental study was conducted on human adipocytes isolated from adipose tissue (ex vivo). Initially, adipocytes were isolated from omentum and abdominal fat samples and subsequently cultured using different concentrations of AdipoRon (10, 20, 40, and 80 µM) for a duration of 2 h. Changes in CAV-1 gene expression were evaluated using the reverse transcription quantitative polymerase chain reaction (RT-qPCR) technique, with the human beta-actin gene (ACTB) serving as the reference gene. Results: Adipocytes isolated from human adipose tissue exhibited a basal expression of the CAV-1 gene, which was not significantly altered by the presence of AdipoRon. However, in some patients with obesity, diabetes, and gastrointestinal tumors, exposure to AdipoRon concentrations led to a slight increase in the expression levels of the CAV-1 gene. Conclusions: This study sheds light on the molecular mechanisms mediated by AdipoRon in human adipose cells, offering preliminary insights for its potential application in the treatment of obesity and type 2 diabetes mellitus.
ISSN:1930-7381
1930-739X