Screening and Optimization of IAA Production by PGPR isolated from Rhizosphere of a Pterocarpus marsupium Roxb. and their Effect on Plant Growth

Indole Acetic Acid (IAA) production is important attribute of PGPR that promote plant growth and development. The rhizosphere is hotspot in the soil that harbors PGPR. The present study was aimed with isolation and screening of IAA producing bacteria from the rhizosphere of Pterocarpus marsupium Rox...

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Bibliographic Details
Published in:Current Agriculture Research Journal 2024-04, Vol.12 (1), p.326-338
Main Authors: Randive, Vaishali Sanjay, Agnihotri, Snehal Nitin, Bhagat, Rani Babanrao
Format: Article
Language:English
Subjects:
Acetic acid
Bacteria
Indoleacetic acid
Microorganisms
Optimization
pH effects
Plant growth
Pterocarpus marsupium
Reagents
Rhizosphere
Root development
Screening
Seeds
Synthesis
Temperature effects
Thin layer chromatography
Tryptophan
Variance analysis
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Summary:Indole Acetic Acid (IAA) production is important attribute of PGPR that promote plant growth and development. The rhizosphere is hotspot in the soil that harbors PGPR. The present study was aimed with isolation and screening of IAA producing bacteria from the rhizosphere of Pterocarpus marsupium Roxb. Optimum culture conditions (pH, temperature, incubation period and L-tryptophan concentration for IAA production were studied for selected isolates and their effect on wheat growth and root development was evaluated. Among twenty four IAA producing isolates five isolates (Et1, Rp1, Rp5, Rp6, and Rp9) produced maximum IAA in range of 50-70 μg/mL and was used in optimization studies. Maximum IAA was produced in 96 hours of incubation, at pH 7 and with 0.1mg/mL of L-tryptophan by all five isolates. 30oC is the most suitable temperature for Et1, Rp1, Rp5, Rp9; whereas Rp6 produced nearly same amount of IAA at wide range of temperature 30-35oC (77-84.12 μg/mL) and at pH 7-8 ( 73-74μg/mL). Out of the five isolates, Rp6 exhibits the highest potential, having a maximum IAA of 84.12 μg/mL at 35°C and pH 7. Although tryptophan influences IAA synthesis but at higher concentration of tryptophan inhibits IAA synthesis. To validate the production of IAA, crude extracts were analyzed using thin layer chromatography (TLC). A spot of standard IAA with the same Rf value (0.91) was found to match a specific spot from the crude IAA.
ISSN:2347-4688
2321-9971
DOI:10.12944/CARJ.12.1.26