Wool keratin-based colorimetric detection of organophosphorus pesticides via a multi-enzyme cascade reaction

A simple and sensitive colorimetric method for organophosphorus pesticides (Ops) detection was proposed via a multi-enzyme cascade reaction. The coordination compound of wool keratin and Cu (WK-Cu) was synthesized by self-assembly and used as a key catalyst in the cascade reaction. The enzymatic kin...

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Bibliographic Details
Published in:Journal of food measurement & characterization 2024-05, Vol.18 (5), p.3344-3352
Main Authors: Cao, Mengrui, Xu, Yiwei, Jia, Xupeng, He, Baoshan, Ren, Wenjie, Wei, Min, Suo, Zhiguang, Jin, Huali, Zhao, Wenhong
Format: Article
Language:English
Subjects:
Acetylcholinesterase
Cascade chemical reactions
Catalysts
Chemistry
Chemistry and Materials Science
Chemistry/Food Science
Colorimetry
Coordination compounds
Engineering
Enzymes
Food Science
Keratin
Organophosphorus pesticides
Original Paper
Peroxidase
Pesticides
Phoxim
Self-assembly
Wool
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Summary:A simple and sensitive colorimetric method for organophosphorus pesticides (Ops) detection was proposed via a multi-enzyme cascade reaction. The coordination compound of wool keratin and Cu (WK-Cu) was synthesized by self-assembly and used as a key catalyst in the cascade reaction. The enzymatic kinetics curve was established to explore the catalytic property, and the obtained K m and V max proved that WK-Cu has a good peroxidase-like activity. Ops could inhibit acetylcholinesterase activity and trigger the multienzyme cascades process, resulting in an amplified response signal. The key conditions were optimized, such as enzyme-linked reaction time and the concentration of each reactant in Ops detection. The detection range of phoxim was 10-1000 µg/L with a detection limit of 2.31 µg/L. Compared with other colorimetric detections, the proposed method exhibited a wider detection range and a relatively lower detection limit. In addition, this colorimetric method showed good universality and anti-interference ability for the analysis of phoxim in apple, cabbage and cauliflower samples. Owing to these merits, the proposed method has a good application prospect in Ops detection.
ISSN:2193-4126
2193-4134
DOI:10.1007/s11694-024-02408-x