Gametogenesis in Rhinella bergi (Anura: Bufonidae): Morphological and morphometric analysis

This study aims to characterize the gonads, folliculogenesis, and spermatogenesis processes of male and female Rhinella bergi through anatomical, histological and morphometric analysis. We worked with specimens previously collected in the spring and summer seasons (2014–2015), in which the gonads we...

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Bibliographic Details
Published in:Acta zoologica (Stockholm) 2024-07, Vol.105 (3), p.317-330
Main Authors: Cheij, Esteban, Olea, Gabriela Beatriz, Rodríguez, Florencia, Céspedez, Jorge, Flores Quintana, Carolina
Format: Article
Language:English
Subjects:
Amphibiotic species
anurans
Bufonidae
Dehydration
Females
Folliculogenesis
Gametocytes
Gametogenesis
Gonads
Morphology
Morphometry
Oocytes
Ovaries
reproduction
Reproductive cycle
Reproductive system
Scanning electron microscopy
Spermatogenesis
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Summary:This study aims to characterize the gonads, folliculogenesis, and spermatogenesis processes of male and female Rhinella bergi through anatomical, histological and morphometric analysis. We worked with specimens previously collected in the spring and summer seasons (2014–2015), in which the gonads were studied based on morphological, histological and morphometric analysis. The reproductive system was analyzed using a stereoscopic microscope and a scanning electron microscope (SEM). Histological preparations were made following the conventional protocol of dehydration, inclusion in paraffin and stained with haematoxylin–eosin, Mallory trichrome and PAS histochemical reactions. The anatomical and morphometric characteristics and the gametogenesis in R. bergi agree with what was proposed for other analyzed species of the Bufonidae family. Females with ovaries with oocytes in different stages of maturity and postovulatory females were found. The males presented continuous spermatogenesis. The results of this work serve as a basis for the characterization of the reproductive cycle in R. bergi and, at the same time, provide background information on the analysis of gametogenic activity. Future investigations will be focused on evaluating the reproductive cycle in this species, both in ovaries and testes as well as Bidder's organ, to compare and correlate with the results obtained in this study.
ISSN:0001-7272
1463-6395
DOI:10.1111/azo.12470