Effect of core–sheath bi‐polymeric scaffolds fabricated from acid‐soluble collagen and poly(lactic acid) derivatives on wound healing

• Published in: Journal of applied polymer science 2024-07, Vol.141 (28), p.n/a
• Main Authors: Mukta, Nasima Akter, Ahmed, Samina, Sarwaruddin Chowdhury, A. M., Tareq, Shafi M., Sajib, Abu Ashfaqur, Bashar, M. S., Haque, Papia
• Format: Article
• Language: English
• Subjects: Amino acids; Benzoyl peroxide; Biocompatibility; biopolymers and renewable polymers; Collagen; Contact angle; Electron microscopes; Electrophoresis; fibers; Field emission; Fish skins; Grafting; NMR; Nuclear magnetic resonance; Polyacrylamide; Polylactic acid; Scaffolds; Sheaths; Sodium dodecyl sulfate; Thermogravimetric analysis; thermoplastics; Tilapia; Vinyl acetate; Wound healing
• ISSN: 0021-8995; 1097-4628
• DOI: 10.1002/app.55636

The core–sheath bi‐polymeric scaffold has been proven as an encouraging material based on the requirement of scaffolds. This study aims to prepare electrospun core–sheath scaffolds by using acid‐soluble collagen (ASC) as core material and poly(lactic acid) (PLA) or PLA‐g‐VAc as sheath material to get the most in combination from a hydrophilic and a hydrophobic polymer. ASC is extracted from waste Tilapia fish skin conserving the triple helix structure of the α1 (130 kDa) chain, and a α2 (120 kDa) chain cross‐linked with the β (280 kDa) chain confirmed by amino acid profile, sodium dodecyl sulphate‐polyacrylamide gel electrophoresis. PLA‐g‐VAc is prepared by grafting vinyl acetate (VAc) onto the PLA chain using benzoyl peroxide as the initiator. FT‐IR, 1H NMR, and 13C NMR of PLA‐g‐VAc reveal that grafting occurs between the double bond of VAc and the methine group of PLA. The morphology of the scaffolds is determined by the field emission scanning electron microscope. FT‐IR, thermogravimetric analysis, differential scanning calorimetry, XRD, and water contact angle measurements are used for further characterization of scaffolds. In vivo, cytotoxicity analysis on the Vero cell line exposes that scaffolds are biocompatible. Application of scaffolds to the surgically produced wounding of skin in a rat model followed by histological assay indicates the enhanced properties of core–sheath scaffolds rather than the single polymeric scaffolds. Core‐sheath acid‐soluble collagen and poly(lactic acid) scaffold as a wound dressing.