Development of Twenty Novel Polymorphic Microsatellite Markers and Their Application in Population Genetic Studies of Konosirus punctatus

Konosirus punctatus is an important fishery resource which is distributed from the Indian Ocean to the northwest Pacific. To study the genetic diversity of K. punctatus , we designed forty microsatellite markers based on Illumina and PacBio sequencing data. Twenty of them (50%) were successfully amp...

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Bibliographic Details
Published in:Thalassas : revista de ciencias del mar 2024-06, Vol.40 (2), p.1093-1099
Main Authors: Miao, Zengliang, Jin, Xun, Chen, Shiyi, Zhang, Kun, Li, Jiasheng, Peng, Ying, Huang, Wenhua, Liang, Xudong, Shen, Haodi, Liu, Yifan, Liu, Bingjian
Format: Article
Language:English
Subjects:
Alleles
Coastal Sciences
Design
Earth and Environmental Science
Earth Sciences
Fish & Wildlife Biology & Management
Fisheries
Fishery resources
Freshwater & Marine Ecology
Gene polymorphism
Genetic analysis
Genetic diversity
Genetic markers
Genetic variation
Genetics
Genomes
Heterozygosity
Konosirus punctatus
Marine & Freshwater Sciences
Microsatellites
Oceanography
Polymorphism
Population genetics
Population studies
Populations
Software
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Summary:Konosirus punctatus is an important fishery resource which is distributed from the Indian Ocean to the northwest Pacific. To study the genetic diversity of K. punctatus , we designed forty microsatellite markers based on Illumina and PacBio sequencing data. Twenty of them (50%) were successfully amplified and exhibited polymorphism which were selected for population genetic analyses in three K. punctatus populations from China (Xiamen, Zhoushan, and Laizhou). The number of alleles per locus ranged from 8 to 15, and the values of expected heterozygosity ( He ) and observed heterozygosity ( Ho ) were 0.454 ~ 0.918 (mean = 0.775) and 0.125 ~ 0.958 (mean = 0.706), respectively. The F ST showed that the three geographic populations were moderately differentiated, while Structure and PCoA analysis showed that there was no significant differentiation between pairwise populations. These microsatellite markers will provide a basis for the study of the population genetics of K. punctatus .
ISSN:0212-5919
2366-1674
DOI:10.1007/s41208-024-00712-3