An exactly solvable model for RNA polymerase during the elongation stage

We consider a Markovian model for the kinetics of RNA Polymerase (RNAP) which provides a physical explanation for the phenomenon of cooperative pushing during transcription elongation observed in biochemical experiments on Escherichia coli and yeast RNAP. To study how backtracking of RNAP affects co...

Full description

Saved in:
Bibliographic Details
Published in:arXiv.org 2024-07
Main Authors: Ngo Phuoc Nguyen Ngoc, Belitsky, Vladimir, Schütz, Gunter M
Format: Article
Language:English
Subjects:
E coli
Elongation
Pushing
Ribonucleic acid
RNA
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We consider a Markovian model for the kinetics of RNA Polymerase (RNAP) which provides a physical explanation for the phenomenon of cooperative pushing during transcription elongation observed in biochemical experiments on Escherichia coli and yeast RNAP. To study how backtracking of RNAP affects cooperative pushing we incorporate into this model backward (upstream) RNAP moves. With a rigorous mathematical treatment of the model we derive conditions on the mutual static and kinetic interactions between RNAP under which backtracking preserves cooperative pushing. This is achieved by exact computation of several key properties in the steady state of this model, including the distribution of headway between two RNAP along the DNA template and the average RNAP velocity and flux.
ISSN:2331-8422