Wheat bran submerged fermentation for protease production by a newly Streptomyces sp. isolate: optimization by factorial designs

Agro-industrial residues represent valuable resources that can meet microorganism’s requirements for growth and metabolite production. The goal of the present study is to formulate an optimum submerged fermentation medium for a newly isolated strain of Streptomyces sp. PO3, utilizing wheat bran as t...

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Bibliographic Details
Published in:Biomass conversion and biorefinery 2024, Vol.14 (12), p.13503-13512
Main Authors: Zerizer, Habiba, Boughachiche, Faiza, Kaki, Amel Ait, Rachedi, Kounouz, Okki, Mohamed El Hadef El
Format: Article
Language:English
Subjects:
Agricultural wastes
Biotechnology
Calcium chloride
Energy
Fermentation
Metabolites
Optimization
Original Article
Protease
Renewable and Green Energy
Wheat
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Summary:Agro-industrial residues represent valuable resources that can meet microorganism’s requirements for growth and metabolite production. The goal of the present study is to formulate an optimum submerged fermentation medium for a newly isolated strain of Streptomyces sp. PO3, utilizing wheat bran as the base medium. Initially, the optimal pH, temperature, and wheat bran concentration were determined using the “one variable at a time” method. Subsequently, to enhance production efficiency, additional components were tested using multifactorial statistical approaches. The optimal conditions affecting protease production were determined by a central composite design. Kinetic production was monitored on the optimized medium for 216 h. The highest protease production was achieved through submerged fermentation at pH 9.0, 40 °C, and 25% wheat bran. Three minerals were statistically significant to improve protease production at a p  
ISSN:2190-6815
2190-6823
DOI:10.1007/s13399-024-05612-3