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Effect of thermosonication on peroxidase, pectin methylesterase activities and on bioactive compounds in custard apple juice

Custard apple juice faces long term storage challenges such as pink discoloration and bitterness due to peroxidase activity. Therefore, inactivation of peroxidase becomes a necessity for longer shelf life of custard apple and its application in food preparations. Heat processing has certain pitfalls...

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Published in:Journal of food measurement & characterization 2017-12, Vol.11 (4), p.1623-1629
Main Authors: Dabir, Mugdha P., Ananthanarayan, Laxmi
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description Custard apple juice faces long term storage challenges such as pink discoloration and bitterness due to peroxidase activity. Therefore, inactivation of peroxidase becomes a necessity for longer shelf life of custard apple and its application in food preparations. Heat processing has certain pitfalls such as loss in nutritional contents of custard apple juice, thereby adversely affecting the sensory quality. In the present study, thermosonication of custard apple juice at a constant temperature of 30 ± 1 °C (20 kHz, 67.84 W/cm 2 ) from 0 to 40 min has been studied with respect to peroxidase and pectin methylesterase inactivation. Effect of thermosonication has also been studied on bioactive components such as total phenolics and flavonoids along with enzyme inactivation. Complete inactivation of peroxidase and pectin methylesterase with 0% residual activity was achieved within 40 min and 50 s, respectively. Total phenolics were found to increase from 70.9 ± 1.6 to 81.7 ± 2.5 mg GAE/g. Sonication was found to have no significant effect ( P  > 0.05) on pH, °Brix, titratable acidity. Ascorbic acid content remained relatively unaffected during sonication. Hence, thermosonication may be employed as food processing technique where retention of bioactive components have been achieved along with safety and quality attributes.
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subjects Acidity
Apples
Ascorbic acid
Bioactive compounds
Biological activity
Bitterness
Brix value
Chemistry
Chemistry and Materials Science
Chemistry/Food Science
Deactivation
Discoloration
Engineering
Flavonoids
Food processing
Food quality
Food Science
Fruit juices
Fruits
Inactivation
Information processing
Juices
Original Paper
Pectin
Pectinesterase
Peroxidase
Phenols
Quality management
Sensory integration
Sensory properties
Shelf life
Sonication
title Effect of thermosonication on peroxidase, pectin methylesterase activities and on bioactive compounds in custard apple juice
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