Structural characterization of n-hexadecanoic acid from the leaves of Ipomoea eriocarpa and its antioxidant and antibacterial activities

• Published in: Biomass conversion and biorefinery 2024-07, Vol.14 (13), p.14547-14558
• Main Authors: Ganesan, Theivanai, Subban, Murugesan, Christopher Leslee, Denzil Britto, Kuppannan, Shanmuga Bharathi, Seedevi, Palaniappan
• Format: Article
• Language: English
• Subjects: Acids; Antiinfectives and antibacterials; Antioxidants; Biological activity; Biotechnology; Column chromatography; Columnar structure; E coli; Energy; Ethanol; Nitric oxide; NMR spectroscopy; Original Article; Palmitic acid; Renewable and Green Energy; Structural analysis
• ISSN: 2190-6815; 2190-6823
• DOI: 10.1007/s13399-022-03576-w

The present study was to evaluate the antioxidant and antibacterial activity of n -hexadecanoic acid from the leaves of Ipomoea eriocarpa through aqueous ethanol extraction and further purification by column chromatography. The structure characterization of the isolated compound was elucidated by FTIR, ESI–MS, 1 H-NMR, and 13 C-NMR spectroscopy. The antioxidant activities of n -hexadecanoic acid were as follows: DPPH (30.19–89.13% at 100–500 µg/ml), ABTS (42.18–83.86% at 100–500 µg/ml), reducing power (0.02–0.16% at 100–500 µg/ml), nitric oxide (18.65–73.17% at 100–500 µg/ml), and superoxide (17.18–81.21% at 100–500 µg/ml) respectively. The n -hexadecanoic acid showed that moderate antibacterial activity against S. aureus , B. subtilis , E. coli , and K. pneumoniae was 7.96 ± 0.05 mm, 10.96 ± 0.15 mm, 11.10 ± 0.17 mm, and 11.93 ± 0.11 mm at the maximum concentration of 50 µg/ml. Hence, the good biological activity of the n -hexadecanoic acid from I. eriocarpa could be used as a natural antioxidant and antibacterial agent in the future pharmaceutical industry.