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Traditional Chinese Medicine Clerodendrum japonicum (C. japonicum) Ameliorates the Pulmonary Fibrosis through Inhibiting the TGF-β/Smad3 Signaling Pathway
Clerodendrum japonicum ( C. japonicum ) is a classical Traditional Chinese Medicine (TCM) and has been used to treat the dozens of diseases in China. However, scientific evidence for the pulmonary fibrosis (PF) treatment of C. japonicum used in traditional medicine is lacking. This study evaluated w...
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Published in: | Biology bulletin of the Russian Academy of Sciences 2024-08, Vol.51 (4), p.883-896 |
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Main Authors: | , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites |
Online Access: | Get full text |
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Summary: | Clerodendrum japonicum
(
C. japonicum
) is a classical Traditional Chinese Medicine (TCM) and has been used to treat the dozens of diseases in China. However, scientific evidence for the pulmonary fibrosis (PF) treatment of
C. japonicum
used in traditional medicine is lacking. This study evaluated whether
C. japonicum
ameliorates the PF and the potential molecular mechanism of
C. japonicum
treatment in vivo and in vitro. We used the PF rat model in vivo
.
The lung coefficient, HE and Masson staining were used to evaluate the lung tissues injury. Transmission electron microscope (TEM) was used to evaluate the organelle structural changes. The inflammatory cytokines IL-1β, IL-6, MCP-1 and TNF-α were detected using ELISA assay. ROS, MDA and GSH were measured to explore the oxidative stress. Western blotting, immunofluorescence (IF) and RT-qPCR were performed to observe the expressions of PF and TGF-β/Smad3 signaling pathway related genes and proteins. Firstly,
C. japonicum
could effectively alleviate the PF injury in rat model.
C. japonicum
could effectively inhibit the levels of inflammatory factors in PF animal model, at the same time,
C. japonicum
could reasonably regulate oxidative stress factors in PF animal model. Furthermore, TGF-β/Smad3 signaling pathway was involved in the PF animal model and
C. japonicum
could inhibit the TGF-β/Smad3 signaling pathway in PF treatment effectively.
C. japonicum
might attenuate the PF in the PF rat model in vivo through inhibiting the TGF-β/Smad3 signaling pathway. These findings suggested that
C. japonicum
might act as a promising TCM to treat the PF. |
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ISSN: | 1062-3590 1608-3059 |
DOI: | 10.1134/S1062359024600028 |