Comparative Study of Dispersive Micro-Solid Phase Extraction of Nandrolone Using MIL-53(Al) and ZIF-8 in Human Plasma

The extraction of nandrolone as a steroid hormone using fast methods is of high importance. This was done by dispersive micro-solid phase extraction in the presence of two widely used organometallic frameworks (MIL-53(Al) and ZIF-8). The extraction steps were optimized by an extensive study on the m...

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Bibliographic Details
Published in:Journal of analytical chemistry (New York, N.Y.) N.Y.), 2024-07, Vol.79 (7), p.901-913
Main Authors: Akbari, Somaye, Takhvar, Azra, Souri, Effat, Ahmadkhaniha, Reza, Morsali, Ali, Khoshayand, Mohammad reza, Amini, Mohsen, Taheri, Alireza
Format: Article
Language:English
Subjects:
Analytical Chemistry
Blood plasma
Chemistry
Chemistry and Materials Science
Comparative studies
Design factors
Design of experiments
Nortestosterone
Solid phases
Sorbents
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Summary:The extraction of nandrolone as a steroid hormone using fast methods is of high importance. This was done by dispersive micro-solid phase extraction in the presence of two widely used organometallic frameworks (MIL-53(Al) and ZIF-8). The extraction steps were optimized by an extensive study on the main factors affecting the absorption/desorption efficiency. The optimum conditions were obtained by design of experiments for the extraction and determination of nandrolone in a blank human plasma matrix. Maximum extraction of nandrolone was achieved in 8.5 min by 2.5 mg of MIL-53(Al) in the solution with 0.89% (w/v) salt concentration, while the maximum extraction by ZIF-8 as another sorbent required 10 min, 4.5 mg of sorbent, and 0.60% (w/v) of salt concentration. Batch experiments were carried out to find appropriate kinetic and isotherm models. Finally, the analytical validation of the proposed method was investigated. The dynamic range of the method was from 0.05 to 1 µg/mL. The precision (expressed as the relative standard deviation, RSD) and accuracy (expressed as the percentage error) of extraction by MIL-53(Al) were found 3.90 and 3.39%, respectively, while those by ZIF-8 were calculated as 4.59 and 4.50%, respectively. The proposed method was successfully applied for the determination of nandrolone in spiked samples, achieving high recovery rates of approximately 96% and 94% using MIL-53(Al) and ZIF-8.
ISSN:1061-9348
1608-3199
DOI:10.1134/S1061934824700278