Incidence and pathological effects during encysted progenetic Euclinostomum heterostomum metacercariae infection in the freshwater fish spotted snakehead {Channa pu net at us

Aim: To assess the incidence and pathological effects during encysted progenetic Euclinostomum heterostomum metacercariae infestation in the freshwaterfish spotted snakehead {Channapunctatus). Methodology: Samples of Channa punctatus were collected from Tilaiya Reservoir, Koderma, Jharkhand, India,...

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Bibliographic Details
Published in:Journal of environmental biology 2024-07, Vol.45 (4), p.446-454
Main Authors: Devnath, S, Kumar, V, Roy, S, Jana, A K, Dhar, S, Bisai, K, Parida, S N, Verma, A, Das, B K, Behera, B K
Format: Article
Language:English
Subjects:
Abundance
Aquaculture
Channa punctata
Degeneration
Fibrosis
Fish
Fishing
Freshwater fish
Gene sequencing
Infections
Infestation
Kidneys
Liver
Monsoons
Morphology
Mortality
Nuclear transformations
Parasites
Pathological effects
Phylogenetics
Phylogeny
rRNA 18S
Sequence analysis
Stains & staining
Wind
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Summary:Aim: To assess the incidence and pathological effects during encysted progenetic Euclinostomum heterostomum metacercariae infestation in the freshwaterfish spotted snakehead {Channapunctatus). Methodology: Samples of Channa punctatus were collected from Tilaiya Reservoir, Koderma, Jharkhand, India, during pre-monsoon, monsoon, and post-monsoon seasons. The encysted metacercariae trematodes were isolated from Channa punctatus. Characterization was performed based on the morphology and sequencing analysis of a partial 18S rRNA gene. Further, the kidney and liver samples from infected fish were subjected to histopathological studies. Prevalence, abundance, and Intensity of infection were further determined. Morphological identification suggests that the encysted metacercariae belonged to Digenean trematodes of genera Euclinostomum, and amplification of partial sequence of 18S rRNA, and phylogenetic analysis revealed the isolated metacercariae were Euclinostomum heterostomum. The histopathological study of the kidney showed hepatocyte degeneration, cytoplasmic vacuolation, and nuclear transformations. Liver fibrosis was observed in Channa punctatus. The prevalence during pre-monsoon, monsoon, and post-monsoon season ranged from 66.1 % to 81.1 %, abundance from 0.74 to 0.92, and intensity ranged from 1 to 1.14 using the Margolisformula. Interpretation: The study highlights the infestation of Euclinostomum heterostomum in Channa punctatus during the pre- monsoon climate appears favorable forthe abundance of parasites. Hence, a proper inspection before consumption is necessary.
ISSN:0254-8704
2394-0379
DOI:10.22438/ieb/45/4/MRN-5244