POLIPEPTIDE „ELASTIN-LIKE". APLICAȚII ÎN DEZVOLTARE DE VACCINURI BAZATE PE PROTEINE RECOMBINANTE

Bacterial cultivation and inducible expression, followed by bacterial lysis and repeated rounds of precipitation/solubilization generated highly purified proteins. V96 and S48I48 were expressed in a bacterial system and were obtained as highly purified protein as a result of several precipitation/so...

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Bibliographic Details
Published in:Romanian archives of microbiology and immunology 2022-11, Vol.81, p.41-42
Main Authors: Vlad - Constantin, Tofan, Cătălin, Ţucureanu, Mădălina, Tălpău, Crina-Georgeta, Stăvaru
Format: Article
Language:eng ; rum
Subjects:
Antigens
Bacteria
Cloning
Cycle protein
Elastin
Lysis
Phase transitions
Polypeptides
Proteins
Solubilization
Vaccine development
Vaccines
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Summary:Bacterial cultivation and inducible expression, followed by bacterial lysis and repeated rounds of precipitation/solubilization generated highly purified proteins. V96 and S48I48 were expressed in a bacterial system and were obtained as highly purified protein as a result of several precipitation/solubilization cycles. Specific phase transition were recorded for V96 at approximately 30°C, associated with aggregation and for S48I48 at approximately 25°C, associated with micelle formation. Generation of fusion proteins was achieved through a Ndel restriction site located at N-terminal of elastin-like polypeptides, which allowed insertion of EGFP and НА-ECTO sequences.
ISSN:1222-3891
2601-9418