DEZVOLTAREA UNUI VACCIN ARN MESAGER - CONSIDERENTE GENERALE

Literature review on the technologies available for the development, production and testing of a messenger RNA vaccine, starting with the establishment of the optimal sequence elements, methods for production and purification of nucleic acids and delivery systems used in vaccination and gene therapy...

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Bibliographic Details
Published in:Romanian archives of microbiology and immunology 2022-11, Vol.81, p.38-39
Main Authors: Cătălin, Tucureanu, Mădălina, Tălpău, Adriana, Costache, Vlad, Tofan, Irina, Ionescu, Ana, Şerbănescu, Crina, Stăvaru, Adrian, Onu
Format: Article
Language:eng ; rum
Subjects:
Antigens
Contaminants
Coronaviruses
COVID-19 vaccines
Double-stranded RNA
Gene therapy
Literature reviews
mRNA
mRNA vaccines
Nucleotide sequence
Purification
Regulatory sequences
Vaccination
Vaccines
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Summary:Literature review on the technologies available for the development, production and testing of a messenger RNA vaccine, starting with the establishment of the optimal sequence elements, methods for production and purification of nucleic acids and delivery systems used in vaccination and gene therapy studies. The mRNA sequence, especially the 5' and 3' untranslated regulatory elements are essential to increase stability and ensure a high level of antigen expression in vitro and in vivo. Reactogenicity and decrease in antigen expression level due to the activation of in vivo antiviral response mechanisms can be diminished by introducing additional purification steps aimed at eliminating DNA, double-stranded RNA and other contaminants resulting from the in vitro transcription processes.
ISSN:1222-3891
2601-9418