Chromatic swept‐source corneal confocal microscopy ‐ A breakthrough innovation?

• Published in: Acta ophthalmologica (Oxford, England) England), 2024-01, Vol.102 (S279), p.n/a
• Main Authors: Sperlich, Karsten, Bohn, Sebastian, Stolz, Heinrich, Guthoff, Rudolf F., Stachs, Oliver
• Format: Article
• Language: English
• Subjects: Confocal microscopy; Cornea; Eye diseases; Microscopy; Morphology
• ISSN: 1755-375X; 1755-3768
• DOI: 10.1111/aos.16516

Methods for visual or quantitative exploration of the cellular morphology of the cornea based on inherent three‐dimensional features open up completely new diagnostic possibilities. However, previous approaches to confocal microscopy of the cornea using mechanical 3D scanning strategies are slow and thus only partially suitable for in vivo investigations. Addressing these shortcomings, the idea of developing a novel confocal microscopic technique for three‐dimensional in vivo imaging of the cellular morphology of the cornea is aimed. By exploiting induced chromatic aberration using a swept‐source laser in conjunction with a specifically designed high‐dispersion optic, an extremely fast method for focal positioning in the tissue shall be developed. Utilizing a dedicated laser line scanning, will enable the display of sectional images in real time. These are comparable to slit lamp images, but with cellular resolution. Furthermore, with an x–y scanning protocol, corneal volumes can be acquired in less than 1 s. The project thus paves the way for cellular 3D slit‐lamp microscopy and, in perspective, provides ophthalmologists with further methods for dedicated diagnostics of ocular surface diseases. This paper summarizes the concept of this novel approach.