Enterococcus faecalis provides protection during scavenging in carrion crow (Corvus corone)

• Published in: Dōngwùxué yánjiū 2024-05, Vol.45 (3), p.451-463
• Main Authors: Hu, Bin, Wang, Jia-Min, Zhang, Qing-Xun, Xu, Jing, Xing, Ya-Nan, Wang, Bo, Han, Shu-Yi, He, Hong-Xuan
• Format: Article
• Language: English
• Subjects: Abundance; Adaptability; Animal models; Bacteria; Biomarkers; Bird migration; Birds; Carrion; Comparative analysis; Composition; Corvus corone; Cytokines; Diet; Ecosystem services; Enterococcus faecalis; Hosts; Immunoregulation; Interleukin 6; Intestinal flora; Intestinal microflora; Microbiota; Microorganisms; Necrosis; Pathogens; Physiology; Relative abundance; rRNA 16S; Salmonella; Scavenging; Technology assessment; Tumor necrosis factor-TNF; Tumor necrosis factor-α; Zoology; γ-Interferon
• ISSN: 0254-5853
• DOI: 10.24272/j.issn.2095-8137.2023.320

The gut microbiota significantly influences host physiology and provides essential ecosystem services. While diet can affect the composition of the gut microbiota, the gut microbiota can also help the host adapt to specific dietary habits. The carrion crow (Corvus corone), an urban facultative scavenger bird, hosts an abundance of pathogens due to its scavenging behavior. Despite this, carrion crows infrequently exhibit illness, a phenomenon related to their unique physiological adaptability. At present, however, the role of the gut microbiota remains incompletely understood. In this study, we performed a comparative analysis using 16S rRNA amplicon sequencing technology to assess colonic content in carrion crows and 16 other bird species with different diets in Beijing, China. Our findings revealed that the dominant gut microbiota in carrion crows was primarily composed of Proteobacteria (75.51%) and Firmicutes (22.37%). Significant differences were observed in the relative abundance of Enterococcus faecalis among groups, highlighting its potential as a biomarker of facultative scavenging behavior in carrion crows. Subsequently, E. faecalis isolated from carrion crows was transplanted into model mice to explore the protective effects of this bacterial community against Salmonella enterica infection. Results showed that E. faecalis down-regulated the expression of pro-inflammatory cytokines tumor necrosis factor alpha (TNF-α), interferon gamma (IFN-γ), and interleukin 6 (IL-6), prevented S. enterica colonization, and regulated the composition of gut microbiota in mice, thereby modulating the host's immune regulatory capacity. Therefore, E. faecalis exerts immunoregulatory and antipathogenic functions in carrion crows engaged in scavenging behavior, offering a representative case of how the gut microbiota contributes to the protection of hosts with specialized diets.